复方红景天制剂对大鼠+10Gz暴露后心肌线粒体ATP酶和呼吸酶链复合物活性的影响  

Effect of Rhodiola Crenulata Compound on Activities of Myocardial Mitochondrial ATPase and Respiratory Chain Complex in Rats after +10Gz Exposure

作　　者：辛益妹[1] 陈良恩[1] 吴峰[1] 赵安东[1] 詹皓[1] 

机构地区：[1]解放军空军航空医学研究所,北京100142

出　　处：《华南国防医学杂志》2013年第5期291-293,304,共4页Military Medical Journal of South China

基　　金：国家自然科学基金项目(81073083)

摘　　要：目的研究复方红景天制剂对大鼠+10Gz(高正加速度)暴露后心肌线粒体三磷酸腺苷(adenosine triphosphate,ATP)酶和呼吸酶链复合物活性的影响。方法 60只雄性健康SD大鼠随机分为5组,即对照组,+10 Gz组,复方红景天制剂低、中、高剂量组,每组12只。复方低剂量组、复方中剂量组和复方高剂量组大鼠分别按0.75 g/kg、1.5 g/kg和3.0g/kg给予复方红景天中药制剂,其余2组动物每天给等量的生理盐水。各组均灌胃给药14天,第15天进行+10 Gz离心机暴露,观察各组动物心肌线粒体ATP酶和呼吸酶链复合物活性的变化。结果与对照组相比,+10 Gz组大鼠线粒体Na+-K+-ATP酶活性以及呼吸链酶复合物Ⅰ、Ⅱ、Ⅳ活性均明显降低(P<0.01或P<0.05)。复方红景天制剂预处理大鼠ATP酶和呼吸链酶复合物活性均升高,复方红景天制剂高剂量组大鼠心肌线粒体Na+-K+-ATP酶、Ca2+-ATP酶和呼吸链酶复合物Ⅰ～Ⅳ的活性均明显高于+10 Gz组(P<0.01或P<0.05)。结论复方红景天制剂对+10Gz暴露后大鼠心肌线粒体损伤有明显的保护作用。Objective To study the effect of rhodiola crenulata compound on the activities of mitochondrial adenosine triphosphate（ATP） and respiratory chain complex in rats after ＋10Gz exposure.Methods Sixty healthy male SD rats were randomly divided into control group,＋10Gz group and low,medium,high dose of compound groups,with 12 rats in each group.The low,medium,high dose of compound groups were fed with rhodiola crenulata compound with the dosage of 0.75 g/kg,1.5 g/kg and 3.0 g/kg respectively once a day for 14 days.The control group and ＋10Gz group were given identical amount of normal saline for 14 days.The rats were exposed to high＋10Gz in the 15th day.The activities of mitochondrial ATPase and respiratory chain complex（CⅠ-Ⅳ） were examined.Results Na＋-K＋-ATPase and CⅠ,CⅡ,CⅣ activities in ＋10Gz group were significantly lower than those in the control group（P0.05 or 0.01）.The activities of Na＋-K＋-ATPase,Ca2＋-ATPase and respiratory chain complex increased after rhodiola crenulata compound preconditioning.ATPase and respiratory chain complex activities of high dose compound group were significantly higher than that of ＋10Gz group（P0.05 or 0.01）.Conclusion Rhodiola crenulata compound has obviously protective effect on the injury of myocardial mitochondria in rats after ＋10Gz exposure.

关 键 词：复方红景天制剂 持续正加速度 三磷酸腺苷酶 呼吸酶链复合物 

分 类 号：R852[医药卫生—航空、航天与航海医学]