黄连素抑制慢性心衰大鼠心肌炎性反应  

Berberine inhibits myocardial inflammation in rat with chronic heart failure

作　　者：何艳[1] 张恩浩[1] 钟国强[1] 曾志羽[1] 李金轶[1] 黎庆捷[1] 

机构地区：[1]广西医科大学第一附属医院心血管内科,广西南宁530021

出　　处：《基础医学与临床》2013年第6期718-721,共4页Basic and Clinical Medicine

基　　金：广西科学研究与技术开发计划项目(桂科攻0816004)

摘　　要：目的在慢性心衰(CHF)大鼠模型检测Toll样受体4(TLR4)的表达,探讨黄连素在慢性心衰炎性反应干预中可能的作用机制。方法大鼠每周腹腔注射阿霉素2.8 mg/kg共10周建立慢性心衰模型,将其随机分为黄连素组、安慰剂组及对照组,每组10只。黄连素组给予黄连素每天21 mg/kg灌胃4周,另两组给予等量0.9%氯化钠注射液。用酶联吸附免疫反应法(ELISA)检测大鼠血清中BNP、TNF-α、IL-1及IL-6的含量;qRT-PCR法检测大鼠血清诱生型一氧化氮合酶(iNOS)表达;HE染色观察大鼠心肌细胞形态学改变;免疫组织化学观察大鼠心肌TLR4蛋白表达和分布。结果黄连素组大鼠血清TNF-α、IL-1、IL-6及iNOS mRNA水平显著低于安慰剂组(P<0.05);黄连素组及安慰剂组TLR4吸光度值均高于对照组,而黄连素组显著低于安慰剂组(P<0.05)。结论黄连素可能通过抑制TLR4信号传导抑制慢性心衰炎性反应。Objective To detect the expression of Toll Like Receptors4 （TLR4） in experimental model rat with chronic heart failure （CHF） , and investigate the possible mechanism of berberinein treating inflammation of CHF. Methods Rat model of CHF was established by intraperitoneal injection of adriamycin with 2.8 mg/kg per week for 10 weeks. The rat model of CHF were randomly divided into berberine group, placebo group and normal group. Each group contained 10 rats. Berberine group was given berberine with 21 mg/kg per day for 4 weeks while other groups were given distilled saline. Serum BNP, TNF-α, IL-land IL-6 were assessed by ELISA, iNOS mRNA was measured by qRT-PCR, TLR4 protein expression and distribution were detected by immunohistoehemistry. HE stain was performed to observe morphological features. Results Serum BNP, TNF-α, IL-1, IL-6 and iNOS mRNA level in berberine group were significantly decreased as compared those in placebo group （P 〈 0. 05 ）. TLR4 Absorbance value in berberine and placebo group were enhanced as compared with normal group （P 〈 0.05 ） , while in berber ine group significantly decreased as compared with in placebo group （ P 〈 0. 05 ）. Conclusions Berberine can in hibit the inflammatory response in CHF may through down-regulation signal transduction of TLR4.

关 键 词：黄连素 慢性心力衰竭 Toll样受体4(TLR4) 

分 类 号：R285.5[医药卫生—中药学]