叶面喷施甲醇和乙醇对万年青生理特性的影响  

Effects of Foliar Spray of Methanol and Ethanol on Physiological Characteristics of Rohdea japonica

作　　者：孙利利[1] 赵艳[1] 周磊[1] 刘昂[1] 王莎莎[1] 肖素勤[1] 陈丽梅[1] 

机构地区：[1]昆明理工大学生命科学与技术学院生物工程技术研究中心,昆明650500

出　　处：《西北植物学报》2013年第3期527-533,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30970263)

摘　　要：为了比较甲醇和乙醇对植物生理特性的影响,分别用5%(V/V)的甲醇和乙醇均匀喷洒于温室条件下盆栽培养的万年青叶片上,以喷施蒸馏水的植物为对照,每周喷施2次,喷施3周后分析这两种醇对万年青叶片生长、生理特性及相关光合基因表达的影响。结果表明,喷施甲醇和乙醇均增加了万年青叶片鲜重、可溶性总蛋白质含量、气孔导度、蒸腾速率、胞间CO2浓度、净光合速率、叶绿素a、b及总叶绿素的含量,降低了过氧化氢的含量,提高了质膜H+-ATPase及H+-泵活性,并且乙醇的作用效果比甲醇更加明显。RT-PCR分析结果表明,光合作用相关基因叶绿素a/b结合蛋白基因(Cab)和铁氧还蛋白-2编码的基因(2-FdO)在处理12h时被甲醇抑制但却被乙醇诱导,72h时被甲醇诱导但却被乙醇抑制;两种醇均诱导光系统Ⅱ反应中心PsbP家族蛋白编码基因(PsbP)的表达。由此推测甲醇和乙醇可能在植物中作为信号分子起作用,且乙醇的刺激效果比甲醇更加显著。In order to compare the effects of methanol and ethanol application on plant physiological charac ters,5% （V/V） methanol and ethanol were sprayed,respectively,on the leaves of Rohdea japonica grown under greenhouse conditions twice a week,with plants sprayed distilled water as the control. After three weeks, the effects of foliar spraying methanol and ethanol on plant growth, physiological characters and the gene expression related photosynthesis were analyzed. These results indicated that methanol and ethanol both increased leaf fresh weight, total leaf soluble protein content, stomatal conductance, transpiration rate, intercellular CO2 concentration, net photosynthetic rate, chlorophyll a, b content and total chlorophyll con- tent ; decreased leaf hydrogen peroxide content ;increased plasma membrane H＋-ATPase and H＋-pump ac- tivity and the effects of ethanol were more significant than those of methanol. RT-PCR showed that at 12 h methanol stimulation repressed the transcription of genes encoding light-harvesting chlorophyll a/b binding protein （Cab） and Ferredoxin-2 （2-FdO）,ethanol stimulation induced their expressions,while up-regulated by methanol and down-regulated by ethanol at 72 h after spraying;both methanol and ethanol induced the transcription of gene encoding the photosystem Ⅱ reaction center PsbP family protein （PsbP）. According to these data,it can be inferred that the two kinds of alcohol may work as a signal molecule in plants,and the stimulating effect of ethanol is more significant than methanol.

关 键 词：甲醇 乙醇 万年青 生理特性 质膜H+-ATPase 

分 类 号：Q945.78[生物学—植物学] Q789