XRCC1和XPD多态性基因型与上皮性卵巢癌铂类药物敏感性的关系  

XRCC1 and XPD genetic polymorphisms predict clinical responses to platinum-based chemotherapy in epithelial ovarian carcinoma

作　　者：王连云[1] 吕杰强[1] 朱雪琼[1] 黄秋穗[1] 陈小鸣[1] 夏露[1] 陈文兵[1] 

机构地区：[1]温州医学院附属第二医院妇产科,浙江温州325027

出　　处：《温州医学院学报》2013年第4期223-227,共5页Journal of Wenzhou Medical College

基　　金：温州市科技计划对外技术交流项目(H20080032);浙江省教育厅科研基金资助项目(20070908)

摘　　要：目的:探讨XRCC1和XPD多态性基因型与以铂类为基础的上皮性卵巢癌化疗敏感性之间的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析方法,检测85例术后以铂类药物为基础联合化疗达6个疗程以上的上皮性卵巢癌患者XRCC1和XPD的多态性基因型,并比较不同基因型与化疗敏感性之间的关系。结果:化疗敏感组和不敏感组间,XRCC1 Arg399Gln基因野生型AA和杂合型AG比较,两者差异有统计学意义(P<0.05),杂合型AG与野生型AA相比,化疗敏感性增加了5.44倍(P<0.05,95%CI为1.78～16.65);纯合突变型GG与野生型AA相比,化疗敏感性增加了5.31倍(P<0.05,95%CI为1.50～18.84)。XPD Lys751Gln基因杂合突变型基因型LG和野生基因型LL相比,LL基因型是LG基因型化疗敏感性的2.88倍(P<0.05,95%CI为1.11～7.49)。结论:XRCC1 Arg399Gln和XPD Lys751Gln基因型可能与上皮性卵巢癌铂类药物化疗的敏感性有关。Objective： To explore the relationship between polymorphisms in XRCCI, XPD and the sensitivity to platinum-based chemotherapy in epithelial ovarian carcinoma （EOC）. Methods： Eighty-five subjects who received primary surgery and more than 6 courses of platinum-based combination chemotherapy and the genetic polymorphism of XRCC1, XPD was examined using polymerase chain reaction-restriction fragment length polymorphism analytical method. Results： The relationship between the genotype of XRCC1 Arg399Gln, XPD Lys751Gln and sensitivity of chemotherapy, between chemosensitivity group and resistance group, the difference between XRCC1 Arg399Gln AA, AG, GG and XPD Lys751Gln LL, LG was significant （P 〈0.05）. To compare XRCCI Arg399Gln AA genetype with XRCC1 Arg399Gln AG genetype, the difference was significant （P〈0.05）; To compare XRCC1 Arg399Gln GG genetype with XRCC1 Arg399Gln AG gene type, the chemosensitivity of XRCC1 Arg399GInGG gene type was 5.31 times （P〈0.05, 95% CI is 1.50-18.84） of XRCC1 Arg399Gln AA gene type. the XRCC1 Arg399GInLG gene type was 5.44 times （P〈0.05, 95% CI is 1.78-16.65） of XRCC1 Arg399Gln AA gene type. And the chemosensitivity of XPD Lys751Gln LL gene type was 2.88 times of XPD Lys751Gln LGgene type （P〈O.05, 95% CI is 1.11-7.49）. Conclusion： Results show that XRCC1 and XPD gene type may be related to platinum-based chemotherapy sensitivity in epithelial ovarian carcinoma.

关 键 词：上皮性卵巢癌 化疗敏感性 CA125 XRCC1 XPD 

分 类 号：R711.75[医药卫生—妇产科学]