2009年广州人肠道病毒EV71型分离株重组分析  

Recombination Analysis of Enterovirus 71 Strain Isolated in Guangzhou,2009

作　　者：关琳琳[1,2] 汪杨[1,2] 罗燕芬[1,2] 徐霖[2,3] 张定梅[1,4] 曹开源[1,2,3,4] 

机构地区：[1]中山大学临床检验标准化研究中心,广州510080 [2]中山大学中山医学院微生物教研室,广州510080 [3]广东省重大传染病预防和控制中心,广州510080 [4]中山大学热带病防治研究教育部重点实验室,广州510080

出　　处：《病毒学报》2013年第3期299-303,共5页Chinese Journal of Virology

基　　金：国家重大传染病防治科技重大专项(2012ZX10004-213)

摘　　要：对人肠道病毒EV71型2009年广州分离株Guangzhou09构建序列系统进化树并分析其重组特点。P1、P2和P3区种系发生进化树提示该分离株发生重组,相似性曲线以及bootscan进一步分析显示该分离株于非结构编码蛋白2B片段区(核苷酸位置4 027bp)处存在EV71亚型C4Shanghai-FJ713137与CVA409-HQ728260型间重组。该分离株与中国大陆目前优势株流行趋势一致,是广州首例发生型间重组且由C4亚型及CVA4型发生重组。To describe the recombination features of human enterovirus 71 strain Guangzhou09 isolated in Guangzhou in 2009,the complete nucleotide sequences of Guangzhou09 were analyzed by various of bioin- formatics software. Phylogenetic analysis based on P1, P2 and P3 regions indicated that recombination occurred between EV71 and CVA4. Phylogenetic, similarity plot and bootscan analysis further revealed the recombination between EV71 genotype C strain Shanghai-FJ713317 and CVA4 strain HQ728260 at re- gion 2B was close to the nucleotide position 4 027. This represents the first evidence for intertypic recom- bination between EV71 subtype C4 and CVA4 in Guangzhou.

关 键 词：手足口病 肠道病毒EV71型 重组分析 

分 类 号：R372.2[医药卫生—病原生物学]