对氨基水杨酸钠对锰致大鼠肾抗氧化酶和病理学改变的影响  

Effects of PAS-Na on kidney antioxidase and pathology changes of manganese-exposed rats

作　　者：唐方萍[1] 潘红波[2] 蒙浩洋[1] 黄艳妮[1] 李少军[1] 陈静雯[1] 区仕燕[1] 姜岳明[1] 

机构地区：[1]广西医科大学公共卫生学院卫生毒理学教研室,广西南宁530021 [2]广西医科大学第一附属医院病理科

出　　处：《毒理学杂志》2013年第2期99-103,共5页Journal of Toxicology

基　　金：国家自然科学基金项目(81072320;30760210)

摘　　要：目的观察对氨基水杨酸钠(PAS-Na)对锰致大鼠肾线粒体、微粒体抗氧化酶、病理学改变的影响。方法 50只雄性SD大鼠按体重随机分为对照组、染锰组。染锰组腹腔注射(ip)MnCl2.4H2O 15mg/kg,对照组ip等容量生理盐水,1次/d,每周5 d,连续6周。然后,将染锰组按体重随机分为染锰组、低、中、高(L、M、H)-PAS干预组。L、M、H-PAS干预组大鼠分别背部皮下注射(sc)PAS-Na 100、200和300 mg/kg,对照组、染锰组背部sc等容量生理盐水,1次/d,每周4 d,连续4周。末次干预后72 h,处死大鼠取肾。试剂盒检测肾线粒体、微粒体超氧化物歧化酶(SOD,WST-1法)、谷胱甘肽过氧化物酶(GSH-Px,DTNB比色法)活性,显微镜观察肾组织病理学改变。结果染锰组肾线粒体SOD活性比对照组低,差异有统计学意义(P<0.05)。经4周干预,PAS-Na干预组肾线粒体、微粒体SOD、GSH-Px活性与染锰组比较,差异无统计学意义。肾病理学检查显示,染锰组出现肾小管蛋白管型、肾小管上皮细胞水肿,部分肾小球出现毛细血管扩张,M-PAS干预组蛋白管型等病理改变恢复明显,L-PAS、H-PAS干预组与染锰组比较也有所恢复。结论染锰可抑制大鼠肾线粒体SOD活性,使大鼠出现肾小管蛋白管型、肾小管上皮细胞水肿、肾小球毛细血管扩张,PAS-Na干预可使其病理形态学改变明显好转。Objective To observe the effects of sodium para-aminosalicylic acid （PAS-Na） on manganese （Mn）-exposed rats＇ kidney antioxidase and pathology changes. Methods 50 male SD rats were randomly divided into control group and Mn-exposed group by weight. M n-exposed group received intraperitoneal injection （ip） of MnC12 .4H20 15 mg/kg, while control group ip physiological saline in the same volume, once a day, all at 5 days a week for 6 weeks. Then, Mn-exposed group was randomly divided into Mn-exposed group, low, medium and high dose PAS-Na（L-, M-, H-PAS） treatment group by weight. L, M, H-PAS treatment group respectively received back subcutaneous injection （sc） of PAS-Na 100, 200, 300 mg/kg, while Mn-exposed group and control group received back sc of physiological saline in the same volume, once a day, all at 4 days a week for 4 weeks. 72 hours after the last treatment, the kidneys were dissected and the activity of antioxidant enzymes （SOD, GSH-Px） in kidney mitochondrial and microsome was detected by related kits, and microscopic structure changes of kidney were observed. Results Mn-exposed group＇ s renal mitochondrial SOD activity declined significantly（P 〈 0. 05）. After 4 weeks＇ treatment, the treatment group＇ s activities of SOD and GSH-Px had no statistical significance compared to the Mn- exposed group. Kidney pathology examination showed that the renal protein tubule and cellular swelling had taken place; angiotelectasis appeared in part of glomerular; changes of kidney pathology reduced obviously in M-PAS group compared to the control group, and it also reduced in L-PAS and H-PAS group. Conclusion Mn exposure can inhibit mitochondrial SOD activity, lead to protein cast and cellular swelling in renal tubule, angiotelectasis in part of glomerular in the kidney which can turn better after the treatment of PAS-Na.

关 键 词：对氨基水杨酸钠 锰 大鼠 肾 抗氧化酶 病理学改变 

分 类 号：R114[医药卫生—卫生毒理学]