低氧预适应对自体原位肝移植大鼠TNF-α诱导肝细胞凋亡的保护作用及机制的研究  

Impact and significance of hypoxia preconditioning on apoptosis of hepatic cells induced by TNF-α in orthotopic liver autotransplantation in rats

作　　者：孟中良[1] 刘崇敏 邵清[1] 张培建 周斌[1] 邹润龙[1] 庄卓男[4] 

机构地区：[1]东南大学医学院附属江阴医院普外科,江苏江阴214400 [2]江苏省江阴中医院外科,江苏江阴214400 [3]扬州大学附属第二临床医院普外科研究室,江苏扬州225000 [4]山东大学齐鲁医院肝胆外科,山东济南250000

出　　处：《中国现代普通外科进展》2013年第5期342-346,共5页Chinese Journal of Current Advances in General Surgery

基　　金：扬州市科技计划项目(YZ2008042);江苏省卫生厅科研课题(H200770)

摘　　要：目的:探讨低氧预适应(HP)对自体原位肝移植大鼠肿瘤坏死因子-α(TNF-α)诱导肝细胞凋亡的保护作用以及可能的机制。方法:采用经门静脉灌注法制备大鼠自体原位肝移植模型,将SD大鼠随机分为正常对照组(NC)、自体肝移植(AT)和AT+低氧预适应(HP)3组。HP组术前用8%氮氧混合气体处理90 min。3组分别于术后1,2,12,24 h处死大鼠取肝脏标本,抽血检测肝功能,RT-PCR检测TNF-α、c-JunmRNA表达水平,透射电子显微镜下观察肝细胞的超微结构变化。结果:术后1、2、12、24h,HP组血清ALT,AST水平较AT组均显著降低,但均高于NC组(P<0.01);HP组各时段TNF-αmR-NA的转录水平较AT组显著降低,但高于NC组(P<0.05);HP组1 h,2 h,12 h时段c-Jun mRNA的转录水平较AT组显著降低,但高于NC组(P<0.05),24 h时段AT、HP、NC组间无显著差异(P>0.05);透射电镜下AT组肝细胞出现典型的凋亡征象,而HP组肝细胞无明显凋亡形态。结论:HP对移植所致的肝脏缺血再灌注损伤有保护作用,其机制可能与减少上游TNF-α表达,抑制JNK信号通路的激活,减轻肝细胞凋亡有关。objeetive： To explore the Impact and significance of hypoxia preconditioning on apoptosis of hepatic cells induced by TNF- α in orthotopic liver autotransplantation in rats. Meth. ods： The model of orthopotic liver transplantation via Portal Vein Perfusion in Rats was established, and the Sprague Dawley rats were randomly divided into the following three groups： normal control （NC）group,autotransplantation （AT）group,and hypoxia preconditioning （HP） group. HP Group was given an 8% oxygen mixed gas for 90 minutes before the operation. Atl,2,12and 24 hours after the operation, the ras were killed and the following tests were conducted： 1）blood was drawn to conduct a chemical examination; 2）RT-PCR observe the expression of TNF- α mRNA and c-Jun mRNA; 3） the ultrastructure changes of hepatocytes were observed under light microscope and transmission electron microscopy.

关 键 词：肝移植 低氧预适应 TNF-Α JNK 缺血再灌注损伤 

分 类 号：R567.3[医药卫生—呼吸系统]