SOCS3和Th1/Th2相关细胞因子在非酒精性脂肪性肝病中的表达及意义  

Expression of SOCS3 and Cytokines of Th1/Th2 in Patients with Nonalcoholic Fatty Liver Disease

作　　者：何蓓晖[1] 陈芝芸[1] 严茂祥[1] 陈瑜[2] 

机构地区：[1]浙江省中医院消化研究室,310006 [2]浙江大学医学院附属第一医院,310003

出　　处：《医学研究杂志》2013年第5期96-99,共4页Journal of Medical Research

基　　金：浙江省医药卫生科技计划基金资助项目(2011KYB058)

摘　　要：目的研究SOCS3和Th1/Th2相关细胞因子在非酒精性脂肪性肝病(NAFLD)中的表达及其相互关系和临床意义。方法取30例NAFLD患者(NAFLD组)和30例健康体检者(对照组)为研究对象,分别应用实时荧光定量PCR法(RT-PCR)法和ELISA法检测外周血SOCS3mRNA和Th1/Th2相关细胞因子IFN-γ、IL-4的表达,观察并比较两组之间的差异。结果 NAFLD组SOCS3mRNA的表达较对照组显著升高(P<0.01);NAFLD组IFN-γ的水平较对照组明显升高(P<0.01),而IL-4的水平则无明显变化(P>0.05)。结论 NAFLD患者外周血Th1型细胞因子占优势,Th1/Th2失衡,IFN-γ的大量表达导致炎症反应,进而促进了NAFLD的发生发展;SOCS3可能是导致NAFLD进展的危险因素。Objective To observe the expression of SOCS3, IFN -γ and IL - 4 in serum of patients with nonalcoholic fatty liver disease, and to discuss the association between them. Methods The expression of IFN -γ and IL - 4 in serum of thirty patients with nonal- coholic fatty liver disease and in thirty healthy adults were detected by ELISA technique. SOCS3 mRNA levels in blood mononuclear cells were analyzed by RT - PCR technique. Rusults The levels of IFN -γ and SOCS3 mRNA of NAFLD group were obviously higher than those of control group（ P 〈 0.01, P 〈 0.01, respectively） , but the level of IL -4 was not different between the two groups. Conclusion Thl is primary at the early satge of NAFLD. SOCS3 is a risk factor of NAFLD.

关 键 词：非酒精性脂肪性肝病SOCS3 TH1 TH2 IFN-γ IL-4 

分 类 号：R581.106[医药卫生—内分泌]