戊型肝炎病毒基因型与戊型肝炎患者临床表现的分析  

Analysis of Genotype and Clinical Manifestations in Patients with Hepatitis E

作　　者：徐斌[1] 惠威[1] 王征[1] 何佳丽[1] 魏琳琳[1] 窦爱华[1] 郭新会[1] 

机构地区：[1]首都医科大学附属北京佑安医院肝病内分泌科,100069

出　　处：《医学研究杂志》2013年第5期132-134,共3页Journal of Medical Research

基　　金：首都医科大学基础和临床课题(2010JL10)

摘　　要：目的观察不同基因型(1型或4型)的戊型肝炎病毒感染的急性戊型肝炎患者临床表现、血清生化指标以及临床转归是否存在差异。方法收集酶联免疫吸附实验方法检测抗HEV IgM阳性,并用简并引物进行反转录巢式PCR方法扩增HEV ORF2的部分基因序列,经核酸序列分析进行基因型分析的急性戊型肝炎患者病例92例。结果 92例HEV RNA阳性的急性戊型肝炎患者中感染HEV1型21例、HEV4型71例。比较两组不同基因型的戊型肝炎患者的临床表现发热、消化道症状等与血清生化检查,均无显著性差异。结论不同基因型的戊型肝炎病毒感染患者临床表现没有差异。Objective To observe whether the different genotypes （type 1 or type 4） are different in patients with acute hepatitis, such as serum biochemical parameters, clinical performance, and clinical outcomes. Methods A total of 92 patients with acute hepatitis by enzyme -linked immunosorbent assay method detect anti HEVIgM positive. HEV ORF2 partial gene sequences was amplified by reverse transcription - nested PCR method using degenerate primers. Nucleic acid sequence analysis was used to genotype analysis. Results Of 92 cases HEVRNA - positive patients with acute hepatitis, 21 cases of the infection HEV1 type, HEV4 71 cases. Compare two groups of different genotypes of hepatitis E in patients with clinical manifestations of fever, gastrointestinal symptoms and serum biochemical tests, there was no significant difference. Conclusion No difference in different genotypes of hepatitis E virus infection in patients with clinical manifestations.

关 键 词：病毒性肝炎戊型 基因型 核酸 序列 

分 类 号：R512.63[医药卫生—内科学]