鼻康片对变应性鼻炎小鼠模型Th1/Th2平衡和血清总IgE的影响  

Effects of Bikang Tablets on Th1/Th2 Balance and IgE in Murine Model of Allergic Rhinitis

作　　者：黄水仙[1] 李勇[1] 谢民强[2] 许熠铭[2] 江珊珊[2] 沈琴[1] 

机构地区：[1]杭州市第一人民医院耳鼻喉科,310006 [2]南方医科大学珠江医院,510282

出　　处：《医学研究杂志》2013年第5期160-163,共4页Journal of Medical Research

基　　金：浙江省中医药科技计划项目(2010A010)

摘　　要：目的研究鼻康片对变应性鼻炎模型小鼠血清总IgE和Th1细胞/Th2细胞(Th1/Th2)平衡的影响,分析中成药鼻康片抗变应性鼻炎的作用机制。方法 24只7周龄Balb/c小鼠随机分成空白对照组(n=6)、模型组(n=6)、西药对照组(n=6)、实验组(n=6)4组。用卵清蛋白致敏建立实验变应性鼻炎模型,西药对照组和实验组分别给予西替利嗪和鼻康片治疗,疗程结束后应用酶联免疫法(ELISA)测定血清总IgE、白细胞介素-4(IL-4)和γ-干扰素(IFN-γ)含量。结果鼻康片可显著降低变应性鼻炎模型小鼠血清IgE和IL-4的水平,与模型组比较有显著性差异(P<0.05)。然而Th1/Th2的比值和IFN-γ的水平明显增高,与模型组比较有显著性差异(P<0.05)。结论鼻康片可通过降低变应性鼻炎模型小鼠血清IgE和IL-4的水平,增加IFN-γ的水平,调节Th1/Th2的平衡来发挥抗变应性鼻炎的作用。Objective To observe the effects of Bikang tablets on Th1/Th2 balance and IgE in mice model of allergic rhinitis, and analyze the reasons for Bikang tablets anti - allergic rhinitis. Methods Twenty four Balb/c mice, 7 weeks old, were randomly divided into four groups：control group, allergic rhinitis model group, drug control group, treatment group. Allergic rhinitis models in mice were established by using ovalbumin intraperitoneal immunization and nasal antigen challenge. Following the allergic rhinitis model successfully prepared, Bikang tablets were used to intervene with modeling animals for two weeks, with xitiliqin being taken as the controlling reagent. By the end of experimental period, serum samples were taken from the animals in each group to detect the levels of total IgE, IL - 4 and IFN - γ, with a comparative analysis among every groups to see the differences in these indicators. Results The serum levels of total IgE and IL - 4 were significantly lower in Bikang tablets treating group than those in modeling group（P 〈 0.05 ）, while the serum levels of IFN -γ and Thl/Th2 were significantly higher in Bikang tablets treating group than in modeling group（P 〈 0. 05）. Conclusion Bikang tablets can decrease the serum levels of IgE and IL - 4,and improve the serum levels of IFN - γ and Thl/Th2. It should be possible for Bikang tablets to bring about the therapeutic effect on allergic rhinitis through its regulatory mechanism to recovery the balance of cytokines between the two sets of lymphocytes Thl and Th2.

关 键 词：变应性鼻炎鼻康片动物模型 白细胞介素-4 IFN-γ IGE 

分 类 号：R289.5[医药卫生—方剂学]