JNK通路介导LPS诱导脐静脉内皮细胞PD-L1表达的实验研究  

JNK pathway regulates LPS-induced PD-L1 expression in the human umbilical vein endothelial cells

作　　者：龙日[1] 刘映峰[1] 严全能[1] 吕自明[1] 缪绯[1] 梁欣伟[1] 

机构地区：[1]南方医科大学珠江医院心血管内科,广东广州510282

出　　处：《热带医学杂志》2013年第5期546-548,569,共4页Journal of Tropical Medicine

基　　金：广东省自然科学基金(10151051501000038)

摘　　要：目的探讨脂多糖(LPS)刺激脐静脉内皮细胞表达程序性死亡因子配体1(PD-L1)与C-JUN氨基末端激酶(JNK)信号通路的关系。方法体外培养脐静脉内皮细胞,按2×107/ml种植在6孔培养板中,在无血清培养基中静止24h后随机分成阴性对照组、LPS组及SP600125+LPS组,其中阴性对照组不加干预因素;LPS组先用DMSO(0.1%V/V)作用1h,再用LPS(1.0μg/ml)刺激24h;SP600125+LPS组先用SP600125(20μmol/L)作用1h后再用LPS刺激24h,Western-blot测定各组PD-L1及P-JNK蛋白表达。结果 SP600125+LPS组PD-L1蛋白表达与对照组比较差异无统计学意义(P>0.05);而SP600125+LPS组和对照组PD-L1蛋白表达与LPS组比较均明显减少,差异均有统计学意义(P<0.05)。SP600125+LPS组细胞P-JNK蛋白表达与对照组和LPS组比较均减少,差异有统计学意义(P<0.05)。结论 LPS可能通过JNK信号通路调控脐静脉内皮细胞表达PD-L1。Objective This study aims to investigate the involvement of JNK in lipopolysaccharides （LPS）-induced programmed death receptor-ligand 1 （PD-L1） expression in human umbilical vein endothelial cells （hUVECs）. Methods UVECs were plated in triplicate at 2×10^7 cells/well of a 6-well plate and incubated in serum-free medium. After hUVECs were cultured for 24 h, the cells were randomly divided into 3 groups： negative control group, cells were treated without the intervention; LPS group, cells were treated with DMSO （0.1%V/V） for lh and then stimulated with 1.0 μg/ml LPS for 24 h;SP600125 and LPS group,hUVECs were treated with SP600125 （20 μmol/L） for lh and then stimulated with 1.0 μg/ml LPS for 24 h. Western-blot was employed to analyze the expression of PD-L1 and P-JNK at protein level. Results The expression of P-JNK in SP600125 and LPS was significantly lower than that in control group and LPS group. The expression of PD-L1 in SP600125 and LPS stimulated group demonstrated no significant difference as compared to control group（P〉0.05）,while the PD-L1 expression in SB600125 and LPS stimulated and control group was significantly lower than LPS group （P〈0.01）. Conclusion These results suggest that activation of JNK pathway as important mechanism for LPS-induced PD-L1 expression on the human umbilical vein endothelial cell.

关 键 词：程序性死亡因子配体1 JNK信号通路 SP600125 脐静脉内皮细胞 

分 类 号：R543.5[医药卫生—心血管疾病]