脂多糖诱导的人气道上皮细胞Caspase-1的表达研究  

Expression of Caspase-1 in human bronchial epithelial cells induced by lipopolysaccharide (LPS)

作　　者：席云祝[1] 于化鹏[1] 樊慧珍[1] 邓火金[1] 夏虎[1] 龚雨新[1] 蔡绍曦[2] 

机构地区：[1]南方医科大学珠江医院呼吸内科,广东广州510282 [2]南方医科大学南方医院呼吸内科,广东广州510515

出　　处：《热带医学杂志》2013年第5期566-569,共4页Journal of Tropical Medicine

摘　　要：目的探讨脂多糖(LPS)诱导人气道上皮细胞半胱氨酸天冬氨酸酶-1(Caspase-1)的表达情况。方法体外培养人气道上皮细胞,将其随机分为阴性对照组、LPS组及Caspase-1特异性抑制剂(Ac-YVAD-cmk)+LPS组(简称cmk+LPS组)。采用四氮唑盐(MTT)法测定各组细胞的增殖能力;实时荧光定量RT-PCR检测各组细胞Caspase-1mRNA的表达水平;Western-blot测定各组细胞Caspase-1蛋白的表达;ELISA法检测各组细胞上清液白介素1β(IL-1β)水平。结果 3组细胞增殖差异无统计学意义(P>0.05);LPS组Caspase-1mRNA的表达水平较cmk+LPS组和对照组增高,3组间差异有统计学意义(P<0.05);LPS组Caspase-1蛋白的表达明显高于对照组和cmk+LPS组,3组间差异有统计学意义(P<0.05);LPS组IL-1β水平高于对照组和cmk+LPS组,3组间差异有统计学意义(P<0.05)。结论 NLRP3炎症复合体诱导的Caspase-1活化参与人气道上皮细胞LPS的致敏过程,并促进下游IL-1β的生成。Objective This study aims to investigate LPS-induced Caspase-1 expression in human bronchial epithelial cells （16HBE）. Methods 16HBE cells were cultured in vitro, the ceils were randomly divided into 3 groups： Negative control group, LPS group and cmk＋LPS group. Cell proliferation was examined by MTT method. The expression of Caspase- 1 mRNA was determined by RT-PCR. The expression of Caspase-1 was examined by Western-blot. The content of IL-1β in the culture supernatant was detected by ELISA. Results There was no difference in the ceUs proliferation among the three groups （P〉0.05）. The expression of Caspase-1 mRNA in LPS-treated 16HBE ceils was significantly higher than the control group and cmk＋LPS group （P〈0.05）. The expression of Caspase-1 （p20） in the LPS group was higher than the control group and cmk ＋LPS group （P〈0.05）. The levels of IL-115 in the culture supernatant from the control group and cmk＋LPS group were lower the LPS group （P〈0.05）. Conclusion NLRP3 inflammasome-induced Caspase-1 activation plays a critical role in the sensitization of human bronchial epithelial cells. It also promotes the production of IL-1β.

关 键 词：人气道上皮细胞 炎症复合体 CASPASE-1 NLRP3 脂多糖 

分 类 号：R562.25[医药卫生—呼吸系统]