SARS冠状病毒N蛋白与γ血红蛋白的相互作用  

Interaction Between SARS-CoV N Protein and γ-Hemoglobin

作　　者：李伟[1,2] 陈婷[2] 李平[2] 曹诚[2] 

机构地区：[1]解放军总医院,北京100853 [2]军事医学科学院生物工程研究所,北京100850

出　　处：《生物技术通讯》2013年第3期325-327,共3页Letters in Biotechnology

摘　　要：目的:研究SARS冠状病毒(SARS-CoV)核衣壳蛋白(N蛋白)与γ血红蛋白的相互作用。方法:利用免疫共沉淀实验验证SARS-CoV N蛋白与γ血红蛋白存在相互作用,并通过Western印迹检测SARS-CoV N蛋白对细胞内γ血红蛋白表达量及稳定性的影响。结果:SARS-CoV N蛋白与γ血红蛋白的相互作用能使SARS-CoV N蛋白抑制γ血红蛋白在293细胞中的泛素化,提高γ血红蛋白在293细胞中的稳定性,从而使γ血红蛋白在细胞内的表达量显著提高。结论:SARS-CoV N蛋白能与γ血红蛋白在细胞内形成复合物,为揭示SARS-CoV在婴幼儿群体中致病率较低的机理提供了实验基础。Objective： To investigate the interaction between SARS-CoV nucleocapsid protein（N protein） and γ-hemoglobin. Methods： The interaction between SARS-CoV N protein and γ-hemoglobin was demonstrated by co-immunopreeipitation, and the expression level and stability of γ-hemoglobin cells influenced by SARS-CoV N protein were investigated by Western blotting. Results： SARS-CoV N protein inhibited ubiquitination of γ-hemoglobin through the interaction, therefore,γ-hemoglobin stability in 293 cell lines was enhanced, as a result, γ-hemoglobin was greatly increased. Conclusion： SARS-CoV N protein and γ-hemoglobin can form complex in cells, which provided the experimental basis to reveal the mechanism of low infection of SARS-CoV in the infants.

关 键 词：SARS冠状病毒 γ血红蛋白 核衣壳蛋白 相互作用 

分 类 号：R392[医药卫生—免疫学] Q78[医药卫生—基础医学]