新型肝穿刺建立原发性肝多房棘球蚴病小鼠模型  

Establishment on mouse model of primary hepatic Echinococcus multilocularisby transhepatic injection

作　　者：卞志远[1] 马少波[1] 夏海洋[1] 赵阶峰[1] 唐景霞[2] 张示杰[1] 彭心宇[1] 杨宏强[1] 

机构地区：[1]石河子大学医学院第一附属医院肝胆外科,石河子832002 [2]石河子大学医学院预防医学系,石河子832008

出　　处：《中国人兽共患病学报》2013年第5期452-455,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金项目(No.81160199);国家科技部支撑计划项目(No.2009BAI82B06)联合资助~~

摘　　要：目的探讨新型肝穿刺方法建立原发性肝多房棘球蚴(Echinococcus multilocularis,E.m)小鼠模型。方法将120只12w龄健康雌性小鼠随机分为切开皮肤经腹壁肌层肝穿刺接种、开腹肝穿刺接种和经皮肝穿刺接种3组,每组40只。20%乌拉坦腹腔注射麻醉后,分别对3组小鼠进行肝脏注射E.m组织混悬液,制备模拟原发性肝多房棘球蚴小鼠模型。结果 3组小鼠的存活率依次为85%、75%、80%(P>0.05),肝脏E.m感染率依次为70.6%、70.0%、62.5%(P>0.05),胸腔误穿率依次为0%、0%、25%(P<0.01)。结论切开皮肤经腹壁肌层肝穿刺接种可成功制备模拟原发性肝多房棘球蚴小鼠模型,既简化了开腹接种的操作,又减少了经皮肝穿刺误穿胸腔的发生率。The purpose of this research was to investigate a new method of transhepatic injection to establish primary E- chinococcus multilocularis （E. m） model in liver of mouse. The 120 healthy female mice of 12 week old were randomly divided into three groups as follows： transhepatic injection through skin incision and muscoli abdominis, laparotomic transhepatic injec- tion, and percutaneous transhepatic injection （40 in each group）. By giving intraperitoneal injection with 20% Urethane, every mouse was injected with E. m tissue suspension respectively through three different operational methods to establish mimic pri- mary E. m model in liver of mouse. Results showed that the survival rates of mice of the three groups were 85%, 75% and 80%, respectively （P〉0.05）;

关 键 词：多房棘球蚴 动物模型 肝脏 肝穿刺 

分 类 号：R383[医药卫生—医学寄生虫学]