潜在血源传播梅毒患者血清抗体检测方法的筛选  

Screening of syphilis serological detection methods in patients with latent blood-transmitted risk

作　　者：朱建锋 卢勤红 熊逸群 孙爱华[3] 

机构地区：[1]宁波市鄞州第二医院,宁波315100 [2]宁波市李惠利医院检验科,宁波3151002 [3]浙江医学高等专科学校,杭州310053

出　　处：《中国人兽共患病学报》2013年第5期514-515,共2页Chinese Journal of Zoonoses

基　　金：浙江省卫生高层次创新人才培养工程项目;浙江省医药卫生科学研究基金(No.2004A018)联合资助~~

摘　　要：目的筛选潜在血源传播梅毒患者血清抗体检测方法。方法采用回顾性研究,以市售ELISA试剂盒和rT-pN15-17-47-ELISA对24 580例潜在的血源传播患者作梅毒筛选,再对上述任一试验结果阳性者血清行ELISA、rTpN15-17-47-ELISA双孔重复试验与TPPA确诊检测,确定ELISA法和rTpN15-17-47-ELISA法的灵敏度;同时以ELISA法和rT-pN15-17-47-ELISA法检测类风湿性关节炎(RA)、系统性红斑狼疮(SLE)等免疫系统疾病患者和健康人血清标本,以判别上述方法的特异性。结果市售ELISA试剂盒和rTpN15-17-47-ELISA阳性检出率分别为5.9%(1 453/24 580)和6.1%(1501/24 580),ELISA试剂盒和rTpN15-17-47-ELISA的灵敏度分别为97.2%(1 439/1 480)和99.3%(1 470/1 480)。结论对潜在血源传播的梅毒患者以rTpN15-17-47-ELISA具有高灵敏度的特异性且成本低廉,有推广价值。The aim of this study was to select a sensitive and specific method for syphilis screening laboratory tests in pa tients with latent blood transmitted risk. A retrospective analysis was adopted to study the positive rate of 24 580 patients with latent blood-transmitted risk to ELISA kits and rTpN15 17-47 ELISA, and all the positive results were further confirmed by TPPA and repeat the test of EI.ISA kits and rTpN15-17-47-ELISA, respectively. Serum samples from 200 healthy individuals, 60 RA patients or SLE patients were detected by ELISA kits and rTpN15-17 47-ELISA. The results of the positive rate of la-tent blood-transmitted risk byELISA and rTpN15 17-47 ELISA were 5.9 % （1 453/24 580） and 6.1% （1 501/24 580）, re- spectively; the sensitive of all the positive samples were 97. 2%（1 439/1 480） and 99.3% （1 470/1 480） respectively. It＇s sug- gested that rTpN15 17-47 ELISA is a convenient, sensitive and specific method for syphilis screening laboratory tests in pa tients with latent blood-transmitted risk.

关 键 词：血源传播 梅毒 血清学检测 苍白密螺旋体明胶凝集试验 酶联免疫吸附试验 

分 类 号：R377[医药卫生—病原生物学]