小型猪自体牙周膜干细胞与复合材料修复牙周骨缺损  

Autologous periodontal ligament stem cells combined with composites repair periodontal bone defects in miniature pigs

作　　者：杨斯惠[1] 钟良军[2] 张鹏涛[3] 张远[3] 张源明[4] 马路平[1] 徐艳[5] 

机构地区：[1]新疆医科大学第一附属医院口腔科,新疆维吾尔自治区乌鲁木齐市830054 [2]杭州师范大学临床医学院,浙江省杭州市310012 [3]杭州师范大学附属医院口腔科,浙江省杭州市310015 [4]新疆医科大学第一附属医院心脏中心,新疆维吾尔自治区乌鲁木齐市830054 [5]南京医科大学附属口腔医院,江苏省南京市210000

出　　处：《中国组织工程研究》2013年第16期2851-2858,共8页Chinese Journal of Tissue Engineering Research

基　　金：自治区科技支疆项目(201091144);浙江省教育厅科研项目(Z201122835)~~

摘　　要：背景:研究发现应用细胞与支架材料相结合可达到较理想的牙周骨缺损修复效果。目的:观察小型猪自体牙周膜干细胞联合羟基磷灰石生物陶瓷复合物修复牙周二类骨缺损的效果。方法:共纳入6只贵州小型猪,用台式慢机磨除的方法建立贵州小型猪上下颌骨二类牙周骨缺损模型,骨缺损位于第三前磨牙和第四前磨牙之间,暴露第四前磨牙的近中牙根。实验组应用自体上下颌尖牙牙周膜干细胞复合羟基磷灰石生物陶瓷进行缺损修复;对照组应用单纯羟基磷灰石生物陶瓷进行修复;以未进行任何修复的作为模型组。各组在骨缺损区域表面覆盖口腔生物膜。造模后12周,取各组缺损牙周组织,通过临床观察,头颅螺旋CT平扫及三维重建和苏木精-伊红染色观察牙周骨组织成骨愈合情况。结果与结论:临床观察显示实验组牙周缺损愈合最好。头颅螺旋CT见实验组缺损区骨密度与周围正常牙槽骨密度无明显差异,对照组及模型组缺损区缺损仍清晰可见,呈不完全愈合状态。苏木精-伊红染色见实验组新生牙槽骨已充满骨缺损处,建立了正常的钙化骨结构;对照组新生牙槽骨尚未充满骨缺损处,钙化程度极低;模型组可见大量的胶原纤维,缺损区仍大量空缺,未见明显新骨形成。可见牙周膜干细胞联合引导组织再生能有效提高小型猪牙周二类骨缺损愈合和牙周再生能力,修复牙周骨缺损。BACKGROUND：Studies have proved that autologous periodontal ligament stem cel s combined with scaffold materials can achieve better effect on the repair of periodontal bone defects. OBJECTIVE：To observe the effect of miniature pig autologous periodontal ligament stem cel s combined with hydroxyapatite bioceramic composites in the repair of category Ⅱ periodontal bone defects. METHODS：Six Guizhou miniature pigs were included, and were used to establish the miniature pig models of upper and lower jaw category Ⅱ periodontal bone defects. The bone defects were located between the third premolar and fourth premolar, and the near root of fourth premolar was exposed. The defects in the experimental group were repaired with periodontal ligament stem cel s obtained from the autologous maxil ary and mandibular canine and combined with hydroxyapatite bioceramic;the defects in the control group were repaired with hydroxyapatite bioceramic simplely;and the model group did not repaired. The defects in each group were covered with oral biofilm. At 12 weeks after modeling, the defect periodontal tissues were obtained from each group, and then the osteogenesis healing of the periodontal bone tissue was observed through clinical observation, heal spiral CT scanning, three-dimensional reconstruction and hematoxylin-eosin staining. RESULTS AND CONCLUSION：Clinical observation showed that healing of periodontal defects in the experimental group was the best. Head spiral CT scanning showed that there was no significant difference of bone mineral density between bone defect area and surrounding normal alveolar bone, and the defects in the control group and the model group were stil clear visible in the incomplete healing state. Hematoxylin-eosin staining showed that the defect area in the experimental group was fil ed with newborn alveolar bone completely, and the normal calcified bone structure was established;in the control group, the defect area was fil ed with newborn alveolar bone incompletely with low calcification degr

关 键 词：生物材料 组织工程口腔材料 干细胞移植 牙周膜干细胞 引导组织再生 成骨 牙周再生 骨缺损 牙周病 省级基金 

分 类 号：R318[医药卫生—生物医学工程]