同种异体脱蛋白骨复合纤维蛋白胶体内构建组织工程骨  

Allogenic deproteinized bone combined with fibrin glue constructs tissue engineered bone in vivo

作　　者：缪旭东[1] 裴国献[2] 闫乔生[1] 贾晶[1] 许有[1] 

机构地区：[1]解放军第五医院骨科,宁夏回族自治区银川市750004 [2]解放军第四军医大学西京骨科医院,陕西省西安市710032

出　　处：《中国组织工程研究》2013年第16期2869-2873,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:已有实验证明复合纤维蛋白胶的异体骨在体外培养中更适合种子细胞生长和增殖。目的:观察复合纤维蛋白胶的脱蛋白骨与单纯脱蛋白骨作为骨髓间充质干细胞支架材料的差异。方法:将28只新西兰大白兔随机分为3组,实验组在兔背部植入复合纤维蛋白胶的脱蛋白骨与自体骨髓间充质干细胞,对照组在兔背部植入单纯脱蛋白骨与自体骨髓间充质干细胞,空白组在兔背部植入单纯脱蛋白骨。结果与结论:随着植入时间的延长,各组植入物碱性磷酸酶活性逐渐增强,但实验组强于对照组与空白组(P<0.01)。实验组与对照组术后4,8周均有新骨形成,且实验组成骨量高于对照组(P<0.01),空白组未见新骨形成。表明复合纤维蛋白胶的脱蛋白骨新骨形成能力优于单纯脱蛋白骨,可作为骨髓间充质干细胞的复合型载体构建于组织工程骨。BACKGROUND： Some experiments have demonstrated that allogenic deproteinized bone combined fibrin glue is more suitable for seed cells growth and proliferation in vitro, OBJECTIVE： To observe the difference between allogenic deproteinized bone combined fibrin glue and allogenic deproteinized bone alone as scaffold materials for bone tissue engineering, METHODS： Twenty-eight New Zealand white rabbits were randomly divided into three groups： allogenic deproteinized bone combined fibrin glue composited with mesenchymal stem cells was implanted in experiment group, allogenic deproteinized bone composited with mesenchymal stem cells implanted in contro group, and allogenic deproteinized bone alone implanted in blank group. RESULTS AND CONCLUSION： With the duration of implantation, the alkaline phosphatase activity of eachgroup was increased gradually. The experiment group was higher than control group and blank group in the alkaline phosphatase activity （P 〈 0.01）. New bone tissue formed in both the experiment group and control group at 4 and 8 weeks after implantation, and there were more new bone tissues formed in the experiment group （P 〈 0.01 ）. In the blank group, there was no new bone formed. AIIogenic deproteinized bone combined fibrin glue is more suitable for mesenchymal stem cells than allogenic deproteinized bone alone, and it can be used as a scaffold for bone tissue engineering.

关 键 词：生物材料 组织工程骨材料 同种异体 脱蛋白骨 纤维蛋白胶 骨髓间充质干细胞 骨组织工程 

分 类 号：R318[医药卫生—生物医学工程]