脱细胞近交系微型猪肌腱修复兔跟腱缺损  

Acellular tendon of mini-pig inbred-lines is used for Achilles tendon repair in a rabbit model

作　　者：靳宪辉[1] 张庆胜[1] 杨建博[1] 刘桂花[1] 魏巍[1] 高春光[1] 林月秋[2] 

机构地区：[1]衡水市哈励逊国际和平医院骨科,河北省衡水市053000 [2]解放军昆明总医院全军骨科中心,云南省昆明市650032

出　　处：《中国组织工程研究》2013年第16期2968-2972,共5页Chinese Journal of Tissue Engineering Research

基　　金：成都军区科研项目(MB07028)~~

摘　　要：背景:异体肌腱移植是目前修复肌腱缺损的理想方法,但移植后的排斥反应是其使用受到限制的主要原因。目的:观察脱细胞处理的版纳近交系微型猪肌腱移植修复兔跟腱缺损的疗效,以及其作为异种肌腱移植支架材料的可行性。方法:将40只日本大白兔制作双后肢跟腱缺损实验动物模型后,随机均分为2组,脱细胞猪肌腱组用脱细胞版纳近交系微型猪肌腱修复,自体肌腱组用自体肌腱修复,术后用3-0肌腱线改良HEMI-KESSLER法进行端端原位吻合。结果与结论:①移植后2周内,脱细胞猪肌腱组与自体肌腱组白细胞计数、C-反应蛋白测量结果差异无显著性意义(P>0.05)。②两组移植后局部反应小,伤口一期愈合,屈踝功能恢复正常。③组织学检查均未见明显淋巴细胞浸润,肌腱缝合处胶原纤维相互衔接。结果说明脱细胞版纳近交系微型猪肌腱能成功修复兔跟腱缺损,且具有组织相容性好、移植排斥反应轻的优点。BACKGROUND：Al ogeneic tendon graft is an ideal method to repair tendon defects. But post-transplantation rejections limit the use of al ogeneic tendons. OBJECTIVE：To investigate the effect of acel ular tendon of Banna mini-pig inbred-lines on repair of rabbit Achiles tendon, and then to evaluate the feasibility and validity of porcine-derived xenogeneic graft. METHODS：Forty Japanese white rabbits were enrol ed to prepare Achil es tendon defect models of the both hind legs, and then were randomly divided into two groups：acel ular porcine tendon group treated with acel ular tendon from Banna mini-pig inbred-lines and autologous tendon group treated with rabbit autologous tendon. End-to-end anastomosis was performed with a 3-0 tendon thread using improved HEMI-KESSLER method. RESULTS AND CONCLUSION：Within 2 weeks post transplantation, there were no significant differences in white blood cel count and C-reactive protein level between the two groups （P〉0.05）. Local reactions in the two groups after transplantation were few, the wound healed at stage Ⅰ, and flexor ankle function returned to normal. Histological results exhibited no lymphocytic infiltration, but interconnected col agen fibers at tendon suture site. These findings indicate that acel ular tendons from Banna mini-pig inbred-lines can successful y reconstruct defected rabbit Achil es tendon with good histocompatibility and little rejection.

关 键 词：生物材料 细胞外基质材料 脱细胞 猪 肌腱移植物 异种肌腱 自体肌腱 肌腱缺损 兔 其他基金 

分 类 号：R318[医药卫生—生物医学工程]