分层多功能层层自组装肝素表面修饰技术  

Layer-by-layer self-assembly multifunctional heparin surface modification

作　　者：魏嵬[1] 李国荣[1] 

机构地区：[1]北京协和医学院,阜外心血管病医院,心血管疾病国家重点实验室,北京市100037

出　　处：《中国组织工程研究》2013年第16期2973-2980,共8页Chinese Journal of Tissue Engineering Research

摘　　要：背景:涂层技术可以在不改变基材的前提下改善材料的表面性质,提高血液相容性,从而减少人工植入装置植入后的不良反应。目的:观察分层多功能层层自组装肝素涂层的血液相容性及抗凝特性。方法:利用层层自组装技术组装出有底层和表层两个功能层的多层自组装涂层,表层为多层肝素/Fe3+层层自组装涂层,底层为壳聚糖层,通过共价键固定在钛金属表面,共5层肝素。以组装相同层数的肝素/壳聚糖层层自组装涂层作为多层对照组;单层对照组为肝素和壳聚糖共混溶液的单层涂层;空白对照组为裸钛片。结果与结论:血液相容性测试结果显示,实验制备的多层自组装涂层溶血率低于多层对照组;甲苯胺蓝法测定结果显示实验制备的多层自组装涂层的肝素总量及4,24,48h的肝素溶解释放量均明显高于多层对照组和单纯对照组;同时动态凝血实验显示实验制备的多层自组装涂层抗凝性能最好。可见分层多功能层层自组装肝素涂层具有良好的血液相容性和抗凝血性能。BACKGROUND：Surface coating can improve material surface properties and hemocompatibility without changing the original material, and thus reduce complications resulting from implantable devices. OBJECTIVE：To observe the hemocompatibility and anticoagulation properties of layer-by-layer self-assembly multifunctional heparin coating. METHODS：Layer-by-layer self-assembly was used to form the self-assembled multilayer coating with two function layers on the surface and the bottom. Surface coating was heparin/Fe3＋multi-layer self-assembly coating and the bottom coating was chitosan, both of which were fastened to the surface of titanium using a covalent bond. There were five layers of heparin. The heparin/chitosan layer-by-layer self-assembly coating with the same number of layers was considered as the multi-layer control group, and the single layer coating of heparin and chitosan blending solution was considered as the single-layer control group;the bare titanium pieces were regarded as the blank control group. RESULTS AND CONCLUSION：Examination of hemocompatibility showed that the hemolysis rate of the self-assembled multilayer coating was lower than that of the multi-layer control group;toluidine blue staining showed that the total amount of heparin and the heparin dissolved and released amount at 4, 24 and 48 hours of the self-assembled multilayer coating were significantly higher than those of multi-layer control group and single-layer control group;dynamic clotting experimental results showed the self-assembled multilayer coating had the best anticoagulation properties. The results indicate that layer-by-layer self-assembly multifunctional heparin coating has the excel ent hemocompatibility and coagulation properties.

关 键 词：生物材料 材料力学及表面改性 层层自组装 肝素 壳聚糖 涂层 血液相容性 抗凝血性 移植物 不良反应 

分 类 号：R318[医药卫生—生物医学工程]