云南油菜(Brassica campestris)乙酰辅酶A羧化酶BCCP亚基基因的克隆及序列分析  

Cloning and Sequence Analysis of Acetyl-CoA Carboxylase BCCP Subunit from Yunan Xiao Rapeseed (Brassica campestris)

作　　者：龚莹[1,2] 彭少丹[3] 陈升位[1] 王学军[1] 官春云[4] 林良斌[1] 

机构地区：[1]云南农业大学农学与生物技术学院,昆明650201 [2]云南大学滇池学院,昆明650228 [3]云南农业大学农科基础课程实验教学中心,昆明650201 [4]湖南农业大学农学院,长沙410128

出　　处：《分子植物育种》2013年第3期345-350,共6页Molecular Plant Breeding

基　　金：国家支撑计划(2011BAD35B04);国家863计划(2011AA10A104)共同资助

摘　　要：本研究以云南小油菜为材料,克隆出乙酰辅酶A羧化酶的BCCP亚基基因,并对克隆基因进行生物信息学分析,结果表明云南小油菜BCCP基因含有6个外显子,编码258个氨基酸,有四个功能保守区,第四功能保守区是生物素化功能域,并且RT-PCR结果表明该基因是表达、有功能的。云南小油菜BCCP亚基与甘蓝型油菜Bp4的氨基酸序列相比较,有30个氨基酸发生变化;云南小油菜BCCP基因的ORF与甘蓝型油菜Bp4mRNA的同源性高达96%。Using yunnan Xiao rapeseed as an experimental material, the gene encoding biotincarboxyl carder protein （BCCP） subunit ofacetyl-CoA carboxylase was cloned. Sequence analysis indicated that the BCCP gene from yunnan Xiao rapeseed （ynsr BCCP） should contain 6 exons that encode 258 amino acids, and the predicted peptide contains 4 conservative domains, in which the fourth domain was considered as the function domain for biot- inylation. Furthermore the result of RT-PCR testified that the gene could be expressive and functional. There are 30 amino acids varying between ynsr BCCP subunit and Bp4 from Brassica rurpus as well as 96% homology between the ORFs ofynsr BCCP gene and Bp4 mRNA.

关 键 词：云南小油菜 乙酰辅酶A羧化酶 BCCP亚基 

分 类 号：S565.4[农业科学—作物学]