深低温冻存同种异体皮肤移植中转化生长因子β1的表达  

Expression of transforming growth factor beta 1 of cryopreserved skin allograft

作　　者：曹立颖[1,2] 丁轩玺[1,2] 杨声坪[1,2] 王冠[1,2] 姜明静[1] 张玉兰[3] 王建民[1,2] 

机构地区：[1]兰州大学第一临床医学院,甘肃省兰州市730000 [2]兰州大学第一医院骨科,甘肃省兰州市730000 [3]甘肃中医学院,甘肃省兰州市730000

出　　处：《中国组织工程研究》2013年第18期3231-3238,共8页Chinese Journal of Tissue Engineering Research

基　　金：甘肃省自然科学研究基金项目(1107RJZA215)~~

摘　　要：背景:皮肤移植和创面覆盖是创伤、烧伤治疗的重要措施。目前,同种异体皮仍然是可利用的最佳创面覆盖物。目的:通过检测转化生长因子β1在深低温冻存后的大鼠同种异体皮肤移植后的表达,评估深低温贮存同种异体皮在创面上的应用。方法:取大鼠皮肤保存于低温-20℃(低温保存组)及-80℃深低温(深低温保存组),冻存1周、1,2个月后,皮片分组移植于同种大鼠背部进行实验,并以自体移植大鼠作为对照组。结果与结论:同低温保存组相比,深低温保存组转化生长因子β1的表达被抑制,移植皮肤排斥反应出现时间延迟及存活时间延长,排斥反应评分也较低。同低温冻存相比,深低温冷冻保存能使同种异体移植物的转化生长因子β1抗原表达降低。提示深低温冻存的异体皮作为一种创面临时覆盖物,在存在皮肤组织缺失或皮源不足的情况下具有广阔的应用前景。BACKGROUND： Skin transplantation and wound surface coverage are the most important measures for the treatment of wound and burn. At present, allogenic skin still is the best available wound coverage. OBJECTIVE： To evaluate the application of allogeneic skin in the wound by detecting the expression of transforming growth factor-~l in rat skin allograft under cryopreservation. METHODS： The skin specimens of rats were preserved at -20 ~C （low temperature preservation group） or at 80~C （cryopreservation group） for 1 week, 1 month and 2 months, respectively. Then skin pieces in each group were transplanted into the back of rats. Autologous skin grafting rats were as control group. RESULTS AND CONCLUSION： Compared with low temperature preservation group, the expression of transforming growth factor-131 in the cryopreservation group was inhibited, rejection of skin allograft was delayed and survival time was pr61onged. Histology score of skin rejection was also lower in the cryopreservation group. Compared with low temperature preservation, cryopreservation could reduce expression of antigen of transforming growth factor-131 in skin allograft. The results show that cryopreserved allograft skin as a kind of wound temporary coverage has wide application prospect in case that skin tissue is inadequate or insufficient.

关 键 词：器官移植 组织移植 同种异体皮肤 深低温冻存 转化生长因子Β1 排斥反应 创面覆盖 皮肤移 植 省级基金 

分 类 号：R318[医药卫生—生物医学工程]