标本保存时间和温度对活化部分凝血活酶时间测定的影响分析  

The Effect of the Specimens Saved Time and Temperature on Detecting the Activated Partial Thromboplastin Time

作　　者：冯倩[1] 邓德耀[1] 陈弟[1] 周林华[1] 李丽莉[1] 

机构地区：[1]云南省第二人民医院检验科,云南昆明650021

出　　处：《昆明医科大学学报》2013年第4期108-110,共3页Journal of Kunming Medical University

摘　　要：目的分析标本保存时间和温度对活化部分凝血活酶时间(APTT)测定的影响.方法选择180例门诊患者标本,分3组,用Sysmex CA1500全自动血凝分析仪及其配套试剂,分别检测抗凝血标本全血室温(26℃)放置、离心后在室温(26℃)及冰箱(4℃)保存条件下0,2,4,6,8 h的APTT结果,各结果分别与各组0 h结果比较.结果标本全血室温(26℃)放置,离心后室温(26℃)或冰箱(4℃)放置6 hAPTT结果与0h相比差异有统计学意义(P<0.05).结论在本实验室检测未使用抗凝药物的患者的APTT标本时,离心或未离心的标本在未开盖的试管中保存在4℃或室温的条件下,应在标本采集后4 h内检测.Objective To analyse the impacts of the specimens saved time and temperature on detecting the activated partial thromboplastin time. Methods The 180 outpatients＇ specimens were selected and divided into 3 groups. Anticoagulant samples of whole blood with a room temperature at 26℃, anticoagulant specimens of plasma with a room temperature at 26℃ , and anticoagulant specimens of plasma at 4℃were preserved for 0 hours, 2 hours, 4 hours, 6 hours, and 8 hours respective, and which were detected by the models of Sysmex CA1500 of automated coagulation analyzer and its supporting reagents for APTT, the resuhs of each groups were compared with the results of 0 hours respectively. Result There was statistically significant difference in the results of APTT of 6 hours compared to 0 hours in each groups （P 〈 0.05）. Conclusion The specimens without anticoagulant treatment, which preserved at a conditions of 4℃ or at room temperature, in the cases of not opening the test tube, whatever the centrifugal or not centrifugal. The AFTT should be detected within 4 hours after specimen collection in our laboratory

关 键 词：活化部分凝血活酶时间 时间 温度 

分 类 号：R446.113[医药卫生—诊断学]