miR-145在间充质干细胞来源软骨细胞肥大化中的作用  

Role of miR-145 in mesenchymal stem cells-originated chondrocytes hypertrophy

作　　者：康菲[1] 杨波[1] 闫演飞[1] 康夏[1] 郭洪峰[1] 窦策[1] 董世武[1] 

机构地区：[1]第三军医大学基础医学部人体解剖学教研室,重庆400038

出　　处：《第三军医大学学报》2013年第11期1058-1061,共4页Journal of Third Military Medical University

基　　金：国家科技支撑计划(2010BAI42G01);第三军医大学青年人才创新基金(2011XQN06)~~

摘　　要：目的探讨miR-145对间充质来源软骨细胞肥大化的调控作用。方法用肥大化完全诱导培养基对间充质干细胞进行软骨细胞肥大化诱导,检测肥大化相关基因表达,确定间充质来源软骨细胞肥大化诱导成功。实验分为转染antagomiR-145(miRNA拮抗剂)组和对照组,应用qRT-PCR检测miR-145对软骨细胞肥大化标志基因Ⅹ型胶原(ColⅩ)、成纤维细胞生长因子受体-1(FGFR-1)和基质金属蛋白酶-13(MMP-13)转录水平的影响。结果转染antagomiR-145可以延缓间充质来源软骨细胞肥大化进程。转染antagomiR-145后肥大化软骨细胞的标志物ColⅩ、FGFR-1、MMP-13的表达量与对照组相比显著降低(P<0.05),而ColⅡ的表达与对照组相比显著增多(P<0.05)。结论抑制miR-145的表达能有效地延缓间充质干细胞向肥大化软骨细胞分化进程。Objective To determine the regulating role of miR-145 in the hypertrophy of mesenchymal stem cells （MSCs）-originated chondrocytes. Methods Complete hypertrophy induction medium was employed to induce MSCs to hypertrophic chondrocytes. The expression of genes related to hypertrophy was detected by qRT-PCR to make sure successful induction from MSCs to hypertrophic chondrocytes. The identified hypertrophic chondrocytes were treated with antagomiR-145 transfection （miR-145 antagonist） or without （control cells）. qRT-PCR was used to detect the hypertrophy related genes such as collagen Ⅹ （Col Ⅹ）, FGFR-1 and MMP-13 to make a preliminary evaluation of the effects of miR-145 on chondrocytes hypertrophy. Results Transfection of antagomiR-145 prolonged the process of hypertrophy of MSCs-originated chondrocytes, and resulted in significant reduced expression of Col Ⅹ, FGFR-1 and MMP-13 when compared with that in control cells （P〈0.05）, while that of Col Ⅱ was significantly increased （P〈0.05）. Conclusion Suppressing miR-145 effectively prevents the MSCs-lineage chondrocytes from entering the late phase of differentiation （hypertrophy

关 键 词：间充质干细胞 软骨细胞 肥大化 MIR-145 SOX 9 

分 类 号：R322.71[医药卫生—人体解剖和组织胚胎学] R329.26[医药卫生—基础医学]