心肌梗死后Neurturin动态表达与心脏迷走神经重构的相关性  

Relationship between neurturin dynamic expression after myocardial infarction and cardiac vagal neural remodeling

作　　者：索菲[1] 胡和生[2] 薛梅[2] 王晔[1] 轩永丽[1] 闫素华[2] 

机构地区：[1]山东大学医学院,济南250012 [2]山东大学附属千佛山医院心内科,济南250014

出　　处：《山东大学学报（医学版）》2013年第5期1-5,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81070088);"泰山学者"建设工程专项经费

摘　　要：目的探讨Neurturin(NRTN)在心肌梗死后的动态表达及其与迷走神经重构的关系。方法选用SPF级Wistar健康雄性大鼠54只,随机分为心肌梗死3 d组(n=10)、心肌梗死1周组(n=10)、心肌梗死4周组(n=10)和各自对应的假手术组(n=8)。利用结扎冠状动脉左前降支的方法制备大鼠心肌梗死模型。从各组大鼠梗死周边及左室游离壁(LVFW)取材,采用RT-PCR、Western blot法检测NRTN的mRNA及其蛋白表达,RT-PCR、免疫组织化学法检测迷走神经的表达及支配。结果心肌梗死1周组大鼠梗死周边心肌中NRTN mRNA及蛋白的表达显著增加,且高于心肌梗死3 d组及心肌梗死4周组(P<0.01)。心肌梗死3 d组梗死灶周边ChAT阳性神经纤维密度及mRNA含量低于假手术组,而心肌梗死1周组及4周组ChAT阳性神经纤维密度及mRNA含量明显高于相应假手术组,且高于心肌梗死3 d组(P<0.01)。结论心肌梗死后NRTN的动态表达与迷走神经重构密切相关,通过调节NRTN的表达可能有利于纠正心肌梗死后心脏自主神经失衡并改善预后。Objective To investigate the relationship between the dynamic expression of neurturin （NRTN） after myocardial infarction （MI） and vagal neural remodeling. Methods 54 male SPF Wistar rats were randomly assigned to six groups： 3 days after MI（n = 10）, 1 week after MI（n = 10）, 4 weeks after MI（n = 10） and three corresponding sham- operated groups （n = 8 ）. The left anterior descending coronary artery was ligated to create rat MI models. Heart tissue was sampled from the infarct border and noninfarct left ventricular free wall （LVFW）. RT-PCR and western blot were used to examine NRTN mRNA and protein expressions. RT-PCR and immunohistochemistry were used to measure the expression and innervation of vagal nerve fibers. Results The expressions of NRTN mRNA and protein significantly increased in the infarct border in the 1 week after MI group, which were higher than those in the 3 days and 4 weeks after MI groups（ P 〈 0.01 ）. Moreover, compared with the corresponding sham-operated groups, the ChAT expression decreased in the 3 days after MI group while it significantly increased in the 1 and 4 weeks after MI groups. Conclusion The dynamic expression of NRTN after MI is closely related to the cardiac vagal neural remodeling. Regulating NRTN expression may be a potential therapeutic target for improving the cardiac autonomic nervous imbalance and the prognosis of MI.

关 键 词：心肌梗死 NEURTURIN 迷走神经 心律失常 

分 类 号：R542.2[医药卫生—心血管疾病]