栽培基质对灵芝菌丝体及子实体中灵芝酸含量的影响  

Effects of Culture Medium on Ganoderic Acid Contents in Mycelium And Fruiting Body of Ganoderma Lucidum

作　　者：马红梅[1] 陈永敢[1] 徐小雄[1] 张耀辉[1] 

机构地区：[1]琼州学院生物科学与技术学院,海南三亚572000

出　　处：《安徽农业科学》2013年第9期3798-3799,3960,共3页Journal of Anhui Agricultural Sciences

基　　金：三亚市院地科技合作项目(2011YD01);三亚市院地科技合作项目(2011YD06);三亚重点实验室项目(L1210);海南省教育厅资助项目(Hjkj2012-40)

摘　　要：[目的]研究适宜不同灵芝(Ganoderma lucidum)品种的栽培基质。[方法]以椰子果皮和木屑栽培基质分别培养灵芝5号种和4-3种,分别获取各级菌丝及子实体,通过乙醇浸提液将样品溶出,采用冰醋酸-香草醛-浓硫酸分光光度法测定总灵芝酸的含量。[结果]5号种木屑栽培所得子实体中的灵芝酸高出3级菌丝0.98个百分点,椰皮栽培基质高出3级菌丝1.20个百分点,木屑栽培基质所得子实体中的灵芝酸含量比椰皮栽培要低0.27个百分点;4-3种木屑栽培基质所得子实体中的灵芝酸高出3级菌丝1.24个百分点,椰皮栽培基质高出1.13个百分点,木屑栽培基质所得子实体中的灵芝酸含量比椰皮栽培基质要高0.20个百分点。[结论]菌丝体与子实体中的灵芝酸含量与灵芝栽培品种和栽培基质均有关系。应根据栽培目的,选择灵芝品种和栽培基质。[ Objective ] The aim was to study the suitable culture medium for different C, anoderma lucidum varieties. [ Method ] Mycelium and fruiting body were gathered by culturing number 5 and 4 - 3 strains with coconut husk substitute cultivation and sawdust. Ganoderic acids was determined by spectrophotometric method using glacial acetic acid and vanillin sulfuric acid as color developer. [ Result] The results showed total ganoderic acid of fruiting body in number 5 strain, which was cultured with sawdust, was 0.98% more than that of three levels of myceli- um; coconut husk was 1.20% more than that of three levels of mycelium and ganoderic acid of body fruiting with culture of sawdust was 0. 27% lower than that of coconut husk. Ganoderic acid of fruiting body cultivated by sawdust was 1.24% more than that of three levels of my- celium, and by coconut was more than 1.13% , while ganoderic acid of fruiting body with culture of sawdust was 0.20% more than that of co- conut husk. [ Conclusion] The ganoderic acid content is related with cultivars and culture medium. So people should choose Ganoderma lucid- urn variety and cultivation medium according to cultivation goal.

关 键 词：栽培基质 菌丝体 子实体 灵芝酸 

分 类 号：S646[农业科学—蔬菜学]