衍生化气相色谱法分析降解液中麦草畏含量的研究  

Determination of Dicamba in Degrada tion Liquid by Derivatization Gas Chromatography

作　　者：靳魁奇[1] 刘宇鹏[1] 金文究 孙杨[1] 潘龙[1] 史欣诺[3] 

机构地区：[1]河南大学生命科学学院生物工程研究所,河南开封475004 [2]浙江杭州鑫富药业股份有限公司,浙江杭州311300 [3]中国药科大学生命科学与技术学院,江苏南京211198

出　　处：《安徽农业科学》2013年第9期3864-3865,共2页Journal of Anhui Agricultural Sciences

基　　金：2011年度河南大学青年骨干教师培养计划项目;河南省教育厅自然科学基础研究计划项目(2009B180004)

摘　　要：[目的]建立衍生化气相色谱定量测定麦草畏的方法。[方法]以五氟苄基溴为衍生剂,乙醚为萃取剂,正十六烷为内标物,使用DB-WAX毛细管柱和FID检测器对麦草畏含量进行测定,并首次对微生物降解液中麦草畏含量进行了测定。[结果]在麦草畏浓度为0～240μg/ml时方法的线性回归方程为:y=3.957x+0.015,相关系数为0.999 0,线性关系良好。方法的相对标准偏差为1.51%,平均回收率在98.5%～101.8%,精密度和准确度较好。[结论]该方法适合于微生物降解液中麦草畏含量的测定,能够满足检测工作的要求。[ Objective ] The aim was to establish an analytical method for the determination of dicamba based on derivatization gas chromatog- raphy. [ Method] The content of dicamba was determined by using DB-WAX capillary column and FID detector with PFBBr as derivative a- gent, ether as extraction agent and n-hexadecane as internal standard. [ Result] When the concentration of dicamba was 0 - 240 μg/ml, the linear regression equation was y = 3. 957x ＋ 0. 015, with the linear correlation coefficient of 0. 999 0. The relative standard deviation was 1.51%, and the recoveries ranged from 98.5% to 101.8%, which suggested that there were high precision and accuracy. [ Conclusion] The method was suitable for the determination of dicamba in microbial degradation liquid, and it could satisfy the requirement of determination a- nalysis.

关 键 词：衍生化气相色谱 麦草畏 五氟苄基溴 降解 

分 类 号：S482.4[农业科学—农药学]