微囊藻毒素-LR对小鼠单核细胞和淋巴细胞的影响  

The influence of microcystin-LR on monocytes and lymphocytes of mice

作　　者：黄夏宁[1] 张永路[1] 叶晓艳[1] 肖雯晴[1] 钟晴[1] 谷康定[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院环境医学研究所教育部环境与健康重点实验室,武汉430030

出　　处：《中华预防医学杂志》2013年第6期556-560,共5页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金（20977938）

摘　　要：目的探讨微囊藻毒素-LR（MC—LR）对小鼠血液中单核细胞和淋巴细胞的影响及毒性效应，筛查血液中早期灵敏效应指标。方法1月龄SPF级雄性昆明小鼠按体重采用随机数字表法分为5组，每组7只，分别分为对照组、低剂量组、中低剂量组、中高剂量组、高剂量组，每天给予1次腹腔注射染毒，分别给予生理盐水及3．125、6．250、12．500、25．000μ一、kg剂量的MC—LR。染毒7d后采用放射免疫方法检测重要相关细胞因子，KCl-SDS沉淀法检测淋巴细胞DNA-蛋白质交联（DNAprotein crosslinks，DPC）情况，流式细胞仪检测单核细胞吞噬功能及单核细胞和淋巴细胞的活性氧变化（reactive oxygen species，ROS），并分析其变化情况。结果中低、中高和高剂量MC—LR染毒组小鼠白细胞介素-6[分别为（346．837±25．536）、（360．847±37．886）、（434．245±35．858）pg／ml]均高于对照组[（232．775±32．816）pg／ml]（t值分别为-7．258、-6．760、-10．966，P值均〈0．05）；高剂量组肿瘤坏死因子-d含量[（10．782±0．966）fmol／ml]低于对照组[（16．878±3．378）fmol／m1]（t=4．591，P〈0．05）。中低和高剂量组淋巴细胞DPCI分别为（242．576±7．545）、（241．472±2．793）ng／ml]高于对照组[（228．657±4．130）ng／ml]（t值分别为-4．282、-6．801，P值均〈0．05）；低、中低、中高和高剂量组淋巴细胞DCF荧光强度（依次为3299．37±120．54、3281．38±58．34、3308．06±136．12、3346．92±108．69）均低于对照组（3770．81±131．39）（t值分别为6．995、9．007、6．472、6．577，P值均〈0．05）。低、中低、中高和高剂量单核细胞DCF荧光强度（依次为3271．51±140．79、3270．05±117．92、3326．90±114．39、3292．49±145．97）均低于对照组（3841．72±130．92）（t值分别为7．847、8．584、7．835、7．411，P值均〈0．05）。其他检测指标未见明�Objective To investigate the influence of microcystin-LR （MC-LR） on monocytes and lymphocytes in blood of mice and to find a sensitive index of toxic effects. Methods Specific pathogen free Kunming male mice, aging 1 month-old, were randomly divided into 5 groups by weights, 7 mice for each group. The mice in 5 groups were exposed to MC-LR through intraperitoneal injection at 0,3. 125,6. 250, 12. 500 and 25. 000 μg/kg respectively for 7 days. Then cytokine levels in the serum were measured by radioimmunoassay, DNA-protein crosslinks （DPC） was measured by the SDS/KC1 precipitation technique, and the phagocytosis and ROS of leukocytes were detected by flow cytomet. Results The levels of interleukin 6 in the 6. 250,12. 500 and 25. 000 μg · kg-1 · d-1 dose groups were （346. 837 ±25. 536） , （360. 847 ± 37. 886） and （434. 245 ± 35. 858） pg/ml respectively, which were significantly higher than those in the control group which the value was （232. 775 ± 32. 816） pg/ml （t values were -7. 258, -6. 760 and - 10. 966 respectively, P values were all 〈 0. 05）. While the level of tumor necrosis factoralpha was （ 10. 782 ± 0. 966）fmol/ml in 25 μg · kg-1 · d-1 dose group was statistically lower than it in the control group which the value was （ 16. 878 ± 3. 378 ） fmol/ml （ t value was 4. 591, P 〈 0. 05 ）. The DPC levels of lymphocytes in 6. 250,12. 500 μg · kg-1 · d-1 dose group were （242. 576 ± 7. 545） , （241. 472 ± 2. 793） ng/ml,higher than it in the control group while the value was （228. 657 ±4. 130） ng/ml （t value was -4. 282, -6. 801 ,P values were all 〈0. 05）. The fluorescence intensity of DCF in lymphocytes in the 4 treated groups were separately 3299. 37 ± 120. 54,3281.38 ± 58.34,3308.06 ± 136. 12 and 3346. 92 ± 108.69,all significantly lower than 3770. 81 -± 131.39 in the control group （t values were 6. 995,9. 007, 6. 472 and 6. 577 respectively, and P values were .all 〈 0. 05）. The fluorescence intensity of DCF in monocytes in th

关 键 词：微囊藻毒素类 小鼠 单核细胞 淋巴细胞 细胞因子类 

分 类 号：R1[医药卫生—公共卫生与预防医学]