日本血吸虫重组铁蛋白的表达和疗效考核价值(英文)  

SCHISTOSOMA JAPONICUM RECOMBINANT FERRITIN: EXPRESSION AND ITS IMMUNODIAGNOSTIC POTENTIAL FOR EVALUATION OF CHEMOTHERAPEUTIC EFFICACY

作　　者：舒新华[1] 易新元[1] 张顺科[1] 曾宪芳[1] 

机构地区：[1]湖南医科大学寄生虫学教研室,湖南长沙410078

出　　处：《中国寄生虫病防治杂志》2000年第2期134-138,共5页Chinese Journal of Parasitic Disease Control

基　　金：This project was supported by the grantofNinth Five-year Key Research Project(No:96-06-04-04)

摘　　要：为探索日本血吸虫重组铁蛋白的疗效考核价值。表达、纯化日本血吸虫重组铁蛋白 ( r Sjferritin) ,用免疫印迹法分析该重组蛋白的免疫活性 ;用 r Sjferritin酶联免疫吸附试验检测治疗前、后不同时期慢性血吸虫病病人的特异性 Ig G及肝吸虫、肺吸虫、旋毛虫、囊虫病病人和正常人血清中的交叉抗体。结果显示 ,r Sjferritin具有较好的免疫活性 ;用其检测日本血吸虫的敏感性和特异性与虫源性天然抗原 ( AWA和 SEA)无显著性差异 ,检测治疗后 6个月、1年和 2年慢性血吸虫病病人 Ig G的阴转率分别为 31.8% ,6 3.3%和 90 .5 % ,治疗后 Ig G水平明显下降 ,治疗后 2年与正常人无显著性差异。因此 r Sjferritin可用于诊断血吸虫病 。In order to analyse the immunodiagnostic potential and evaluation of chemotherapeutic efficacy with S. japonicum recombinant ferritin(rSjferritin). S. japonicum ferritin was expressed in E. coli strain ER2566 and purified with HisBind Resin. Immunoreactivity of rSjferritin was analysed by western blot. rSjferritin was used to detect specific IgG in sera of patients before treatment and at 6 months,1 year and 2 years after treatment, and also to detect cross reactive IgG antibody in sera of patients with other parasitic diseases and healthy individuals by ELISA. The results showed that purified rSjferritin was only recognized by schistosomeinfected sera and immunized rabbits sera. The sensitivity and specificity of rSjferritin as coating antigen was as good as those of AWA and SEA. The negative reversion rate of specific IgG in the sera of patients at 6 months, 1 year and 2 years was 31.8%, 63.3% and 90.5% respectively. The level of IgG against rSjferritin significantly decreased after treatment, and at 2 years after treatment, the IgG returned to normal level. rSjferritin could be used to diagnose schistosomiasis japonica and evaluate the therapeutic efficacy of the disease.

关 键 词：日本血吸虫病 重组铁蛋白 疗效考核价值 

分 类 号：R532.210.5[医药卫生—内科学]