哮喘大鼠模型血液系统指标的改变  

Changes of hematological system parameters in allergic asthma model of rats

作　　者：董峰[1] 徐菡[1] 毕鑫星[1] 张洪瑞[1] 王成彬[1] 

机构地区：[1]解放军总医院临检科,北京100853

出　　处：《解放军医学院学报》2013年第7期760-761,778,共3页Academic Journal of Chinese PLA Medical School

基　　金：国家自然科学基金项目(30873419)~~

摘　　要：目的探讨过敏性哮喘大鼠模型血液系统指标的改变。方法将20只SPF级雄性Wistar大鼠随机分为正常组和哮喘组(每组10只)。哮喘组采用卵清蛋白(ovalbumin,OVA)+氢氧化铝凝胶致敏3次后OVA激发1次建模,正常组以0.9%氯化钠溶液(normal saline,NS)为替代品致敏和激发。建模成功后通过血流变、血气及凝血四项指标评价哮喘大鼠血液系统的改变。结果与正常组相比,哮喘组大鼠血浆及全血凝血黏度增加(P<0.05,P<0.01),动脉血氧分压(PaO2)、动脉血氧饱和度(SaO2)显著下降(P<0.05,P<0.05),凝血酶原时间(prothrombin time,PT)和凝血酶时间(thrombin time,TT)延长(P<0.01,P<0.05),纤维蛋白原(fibrinogen,FIB)水平增高(P<0.01)。结论哮喘大鼠模型存在凝血平衡紊乱及血气等指标的改变。Objective To study the changes of hematological system parameters in allergic asthma model of rats.Methods Twenty healthy male Wistar rats were randomly divided into normal control group and asthma model group.Rats in asthma model group were sensitized 3 times with ovalbumin（OVA） and aluminum hydroxide gel and challenged with OVA once,and those in normal control group were sensitized with 0.9% normal saline.Changes of hematological system parameters in the rats were assessed according to their hemorheology,blood gas and blood coagulation.Results The plasma and whole blood viscosity and FIB level were significantly higher,the PaO2 and SaO2 were significantly lower,the PT and TT were significantly longer in asthma model group than in normal control group（P 〈0.01,P 〈0.05）.Conclusion Parameters of blood coagulation and blood gas are changed in allergic asthma model of rats.

关 键 词：哮喘 模型 动物 血液流变学 血气分析 血液凝固 

分 类 号：R562.25[医药卫生—呼吸系统]