SHIP和Caspase-3mRNA在骨髓增生异常综合征患者骨髓组织中的表达及其临床意义  

Expressions of SHIP and Caspase-3 mRNA in bone marrow tissue of patients with myelodysplastic syndrome and their clinical significances

作　　者：贾晓辉[1] 罗建民[2] 刘亚平[1] 尹婉宜[1] 张丽红[1] 

机构地区：[1]河北医科大学第一医院血液内科,河北石家庄050031 [2]河北医科大学第二医院血液内科,河北石家庄050000

出　　处：《吉林大学学报（医学版）》2013年第3期554-558,共5页Journal of Jilin University：Medicine Edition

基　　金：河北省自然科学基金资助课题(H2012206139)

摘　　要：目的:检测骨髓增生异常综合征(MDS)患者骨髓组织中SHIP、Caspase-3mRNA的表达,探讨其表达与MDS发病及向白血病转化过程的关系。方法:根据国际预后评分系统(IPSS)选择62例MDS患者,其中低危(low-risk)组20例、中危1(Int-1)组12例、中危2(Int-2)组13例和高危(high-risk)组17例,采用RT-PCR法检测62例MDS患者及30例健康正常者(对照组)骨髓单个核细胞(BMNC)中SHIP mRNA及Caspase-3mRNA的表达水平,并分析两者之间的关系。结果:MDS低危组和中危1组BMNC中SHIP mRNA表达率(100%,100%)及表达水平(4.467±2.899,4.529±1.975)分别与对照组(100%,4.623±2.979)比较差异均无统计学意义(P>0.05);在中危2组和高危组中,BMNC中SHIP表达率(92%,88%)下降,表达水平(2.124±1.880,2.574±1.807)低于对照组(P<0.05)。Pearson相关分析,MDS患者BMNC中SHIP mRNA与Caspase-3mRNA表达水平呈正相关关系(r=0.714,P<0.05)。随骨髓原始细胞比例升高,SHIP mRNA表达水平降低(F=3.7,P<0.05),不同性别(t=0.940,P>0.05)、不同年龄(t=1.419,P>0.05)患者SHIP mRNA表达水平差异无统计学意义。结论:SHIP在高危MDS患者BMNC中呈低表达或不表达,其表达水平可以作为判定MDS预后的一个指标。Objective To detect the expressions of SHIP mRNA and Caspase-3 mRNA in bone marrow tissue of the patients with myelodysplastic syndrome（MDS） and to discuss the relationship between SHIP mRNA and Caspase-3 mRNA expressions and the occurrence and development of MDS.Methods 20 low-risk MDS cases（low-risk group）,12 Int-1 MDS cases（Int-1 group）,13 Int-2 MDS cases（Int-2 group）,and 17 high-risk MDS cases（high-risk group） were selected according to International Prognostic Scoring Syterm（IPSS）.30 healthy volunteers were regared as control group.The expression levels of SHIP mRNA and Caspase-3 mRNA in bone marrow mononuclear cells（BMNC） in various groups were detected by RT-PCR method,and the relationship between them was analyzed.Results The positive rates of SHIP mRNA were 100% and 100% and its expression levels were 4.467±2.899 and 4.529±1.975 in low-risk group and Int-1 group,respectively,while they were 100% and 4.623±2.979 in control group,so there were no significant differences between low-risk group,Int-1 group and control group（P0.05）.The positive rates of SHIP mRNA were 90% and 88% and the expression levels were 2.124±1.880 and 2.574±1.807 in Int-2 group and high-risk group,respectively,so there were significantly differences compared with control group（P0.05）.The Pearson correlation analysis results showed that the positive correlations were found between the level of SHIP mRNA and the level of Caspase-3 mRNA in MDS patients（r=0.714,P0.05）.The expression level of SHIP mRNA was decreased with the increasing of premitive cells（F=3.7,P0.05）.There were no significant differences of SHIP mRNA expression levels of patients between different genders（t=0.940,P0.05） and different ages（t=1.417,P0.05）.Conclusion The expression level of SHIP is lower or the SHIP doesn＇t express in BMNC of the high-risk MDS patients,and its expression level can be used as an indicator to judge the prognosis of MDS.

关 键 词：骨髓增生异常综合征 基因表达 SHIP 聚合酶链反应 

分 类 号：R551.3[医药卫生—血液循环系统疾病]