京万红软膏治疗糖尿病慢性创面的实验研究  

Therapeutic effects of Jingwanhong ointment(京万红软膏)on healing of chronic diabetic wound in mice

作　　者：姜玉峰 黄沙[2] 邹吉平[2] 许樟荣 高虹[3] 付小兵[2] 

机构地区：[1]解放军第三O六医院全军糖尿病诊治中心,北京100101 [2]解放军总医院第一附属医院全军创伤修复与再生重点实验室,北京100048 [3]天津生物工程职业技术学院,天津300462

出　　处：《感染．炎症．修复》2013年第1期34-37,F0003,共5页Infection Inflammation Repair

摘　　要：目的:探讨京万红软膏促进糖尿病小鼠慢性创面愈合的效果及机制。方法:清洁级、同周龄雄性C57小鼠54只,随机分为京万红软膏组、对照药物组和空白对照组,每组18只。采用连续3 d腹腔注射1%链脲佐菌素60 mg/kg的方法建立糖尿病小鼠模型,然后在小鼠背部制作直径1 cm的圆形全层皮肤缺损创面。创面形成第2日开始,京万红软膏组创面涂抹京万红软膏,厚度0.5 cm;对照药物组创面涂抹相同厚度复方磺胺嘧啶锌凝胶剂;空白对照组不给药。3组创面均以凡士林纱布覆盖,每日换药1次。于给药后第3、7、10、17、21天,用塑料透明膜描记创面大小,计算创面愈合率。于给药后第7、17天各组分别随机选取3只小鼠处死,观察创面及创缘2 mm范围内全层皮肤及肉芽组织的大体形态变化及组织病理学变化。结果:给药后7 d,两药物治疗组创面愈合率均有所升高,但3组间差异无显著性(P>0.05)。给药后10、17和21 d,对照药物组创面愈合率[(81.00±0.85)%,(95.00±0.29)%,(97.00±0.37)%]明显高于空白对照组[(77.00±1.35)%,(87.00±1.17)%,(90.00±0.96)%,P<0.05],而京万红软膏组愈合率[(85.00±1.93)%,(100.00±0)%,(100.00±0)%]明显高于对照药物组(P<0.05)。治疗17 d京万红软膏组创面全部愈合,而对照药物组和空白对照组在治疗后21 d仍未全部愈合。组织病理学观察发现,京万红软膏较对照药物复方磺胺嘧啶锌凝胶剂能更早使炎症减轻、创面上皮化,而且表皮细胞复层分化良好,新生胶原排列整齐,创面组织结构恢复正常化明显,显示具有更好的愈合质量。结论:京万红软膏对于糖尿病慢性创面有良好的促进愈合作用。Objective：To explore the promoting effect of Jingwanhong ointment （京万红软膏） in healing of chronic open wound and its mechanism in diabetic mice. Methods： Fifty-four clean-grade male C57 mice were randomly divided into Jingwanhong ointment group, compound sulfadiazine zinc gel group （medicine control group） and blank control group （n= 18）. The model of diabetes was replicated by consecutive administration of 1 % streptozotocin intraperitoneally 60 mg/kg for 3 days. Then, on the back of mice, round full-thickness skin wounds with diameter of 1 cm were produced. Jingwanhong ointment or compound sulfadiazine zinc gel was applied on the wounds of the two medicine treatment groups after the second day of wound formation, and vaseline gauze was applied on all the wounds of the three groups. Dressing-change was done daily. On the 3rd, 7th, 10th, 17th, 21st days after treatment, the circumference of the wounds was drawn on transparent plastic films for the estimation of the rate of wound healing. On 7th and 17th day after treatment, 3 mice of every group were sacrificed respectively, and gross morphological changes,histopathological changes in skin and granulation tissue within 2 mm of edge of the wounds were examined. Results：The wound healing rate of the two treatment groups increased on the 7th day after treatment, but the difference was not significant among the three groups （P^0.05）. On the 10th, 17th, 21st day after treatment, the wound healing rate in medicine control group [（81.00±0.85）%,（95. 00±0.29）%, （97.00± 0. 37）%] was significantly higher than those of the blank control group [（77.00±1.35）% ,（87.00±1.17） %, （90.00± 0.96） % ,P〈0.05], and that of the Jingwanhong ointment group [（85.00±1.93） %, （100.00±0） %, （100.00±0） % ]was significantly higher than that of the medicine control group （P〈0.05）. The wounds were healed on the 17th day in Jingwanhong ointment group, but not in the medicine controi and blank control groups even on t

关 键 词：京万红软膏 糖尿病溃疡 创面愈合 

分 类 号：R47[医药卫生—护理学]