MiR-143在山羊卵巢组织中的表达及功能分析  

Expression and biological function of MiR-143 in goat ovaries

作　　者：张晓东[1] 凌英会[1] 李运生[1] 张运海[1] 张子军[1] 丁建平[1] 章孝荣[1] 

机构地区：[1]安徽地方畜禽遗传资源保护和生物育种省级实验室安徽省羊繁育工程技术研究中心安徽农业大学动物科技学院,合肥230036

出　　处：《安徽农业大学学报》2013年第3期411-417,共7页Journal of Anhui Agricultural University

基　　金：国家高技术研究发展计划(863)项目(2011AA100307-4);安徽省技术创新工程试点省专项(11Z0101095);安徽省科技计划项目(11010302108)共同资助

摘　　要：旨在分析miR-143在妊娠与非妊娠山羊卵巢组织中的表达情况,并探讨其在山羊卵巢功能发挥以及其他生物学过程中所起的作用。采用Solexa测序及荧光定量PCR技术在妊娠和非妊娠山羊卵巢组织中检测miR-143的表达水平,并对其进行靶基因预测和GO富集分析。Solexa测序在山羊卵巢中检测到miR-143的表达,共发现miR-143及其异构体1 074个,总拷贝数为109 523,平均拷贝数为102;定量结果显示miR-143在妊娠山羊卵巢中的表达量是非妊娠山羊的12倍且差异显著(P<0.05);GO分析表明miR-143广泛参与机体生殖发育、细胞增值分化等多个生物学过程。miR-143在妊娠山羊卵巢中的表达水平显著高于非妊娠山羊,而且其可能在卵巢激素分泌与作用应答、妊娠维持等生殖生物学过程中发挥潜在作用。With the purpose of determining biological role of miR-143 in goat ovaries and many other bio- logical processes, the differential expression of miR-143 between pregnant and non-pregnant goat ovaries was studied by Illumina Solexa sequencing and q-PCR technology, followed by target gene prediction and GO analysis Solexa sequencing indicated that a total of 1 074 miR-143 isomiRs were identified in goat ovaies with 109 523 total read counts, and average expression level was 102. Q-PCR analysis showed that expression level ofmiR-143 in pregnant goat ovaries was 12 times more than that in non-pregnant goat ovaries, and the difference was signifi- cant （P 〈 0.05）. The GO analysis showed that miR-143 played important roles in animal reproduction, cell prolif- eration and differentiation. MiR-143 may play potential roles in animal reproductive biological processes such as ovarian hormone secretion and pregnancy maintenance.

关 键 词：MIR-143 Solexa测序 定量PCR GO分析 山羊卵巢 

分 类 号：S827.2[农业科学—畜牧学]