糖耐康对转化生长因子-β_1诱导的人肾小管上皮细胞纤维化细胞因子的影响  

Effect of Tangnaikang on Fibrosis Cell Factor of Human Renal Tubular Epithelial Cell HK-2 Induced by TGF-β_1

作　　者：骆元斌[1,2] 杨丽霞[1,2] 李晓东[1,2] 程涛[1,2] 刘铜华[3] 吴丽丽[3] 

机构地区：[1]甘肃省中医院 [2]甘肃省中医药研究院,甘肃兰州730050 [3]北京中医药大学,北京100029

出　　处：《中国中医药信息杂志》2013年第6期29-31,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(30973909);北京中医药大学创新团队项目(2011-CXTD-19)

摘　　要：目的观察糖耐康药物血清干预转化生长因子-β1(TGF-β1)诱导的人肾小管上皮细胞(HK-2)纤维化细胞因子的作用,探讨糖耐康防治肾纤维化的作用机理。方法将HK-2细胞用含10%胎牛血清的DMEM/F12(1∶1)培养基培养。实验分为空白对照组、单纯TGF-β1诱导组(TGF-β110 ng/mL)、空白血清对照组(TGF-β110 ng/mL+10%空白血清)、干预1组(TGF-β110 ng/mL+5%糖耐康药物血清)、干预2组(TGF-β110 ng/mL+10%糖耐康药物血清)、干预3组(TGF-β110 ng/mL+20%糖耐康药物血清)。药物干预24 h后,荧光定量PCR检测结缔组织生长因子(CTGF)、纤溶酶原激活物抑制物-1(PAI-1)、基质金属蛋白酶-9(MMP-9)mRNA的表达。结果 HK-2细胞经TGF-β1诱导后,CTGF、PAI-1 mRNA的表达显著上升,而MMP-9 mRNA的表达减弱,与空白对照组比较差异有统计学意义(P<0.05)。经糖耐康药物血清干预后,CTGF、PAI-1 mRNA的表达逐步下降,而MMP-9 mRNA的表达逐步上升,与单纯TGF-β1诱导组比较差异有统计学意义(P<0.05)。结论糖耐康在一定程度上能够抑制TGF-β1诱导人肾小管上皮细胞纤维化,其机制可能与调节纤维化细胞因子表达有关。Objective Through studying the effect of Tangnaikang on the expression of fibrosis cell factor mRNA of human renal tubular epithelial cells HK-2 induced by transforming growth factor-β1（TGF-β1）,to explore the mechanism of Tangnaikang on the prevention and treatment of renal fibrosis.Methods The HK-2 cells were cultured by DMEM/F12（1∶1） with 10% fetal bovine serum and divided into control group,TGF-β1 group（TGF-β110 ng/mL）,blank serum control group（TGF-β110 ng/mL＋10% animal serum）,Tangnaikang drug-containing serum therapy groups（TGF-β110 ng/mL ＋ 5% Tangnaikang or ＋ 10% Tangnaikang or＋20% Tangnaikang）.After 24 h,the expression of CTGF,PAI-1 and MMP-9 mRNA were tested by fluorescence quantitatiye PCR.Results After induced by TGF-β1,the expression of CTGF and PAI-1 mRNA were much increased and the expression of MMP-9 mRNA was decreased in HK-2 cells compared with the control group（P 0.05）.But the expression of CTGF and PAI-1 mRNA were decreased and the expression of MMP-9 mRNA was increased in HK-2 cells cultured with TGF-β1plus Tangnaikang compared with TGF-β1 group（P 0.05）.Conclution Tangnaikang can inhibit the fibrosis of human renal tubular epithelial cells induced by TGF-β1 to some extent,by regulating the expression of fibrosis cell factors.

关 键 词：糖耐康 转化生长因子-Β1 人肾小管上皮细胞 结缔组织生长因子 纤溶酶原激活物抑制物-1 基质金属蛋白酶-9 

分 类 号：R285.5[医药卫生—中药学]