4株海洋酵母菌胞外酶活力及其对水产养殖有机污染物的降解  

Studies on activities of extracellular enzymes of four yeasts and their degradation of organic pollutants from aquaculture

作　　者：戴顺珍[1] 夏新建[1] 许忠能[1] 林小涛[1] 

机构地区：[1]暨南大学水生生物研究所,广东省高校水体富营养化与赤潮防治重点实验室,广东广州510632

出　　处：《生态科学》2013年第1期22-26,共5页Ecological Science

基　　金：汇丰/世界自然基金会华南湿地项目(编号:02-000056);广东省科技计划项目(编号:20108B020309005);广州市科技计划项目(编号:2010Z1-E401);广东省海洋渔业科技推广专项项目(编号A201001H05)

摘　　要：研究利用平板鉴别培养基法观察比较4株海洋酵母菌生淀粉酶、蛋白酶、脂肪酶和纤维素酶等胞外水解酶的能力,并进一步分析测定其酶活性。结果表明,圆丘假丝酵母菌(Candida.colliculosa)DY11-1能产淀粉酶、蛋白酶和脂肪酶,其酶活力分别为181.4 U/mL、123.1 U/mL和36.7 U/mL;毕赤酵母(Pichia sp)DY11-6和近平滑假丝酵母(Candida parapsilosis)DY07-1都能产生淀粉酶和蛋白酶,它们淀粉酶的活力分别为108.1 U/mL和96.5 U/mL,蛋白酶的活力分别是35.1 U/mL和128.4 U/mL;粘红酵母(Rhodotorula glutinis)DY02-3四种胞外酶皆无。以罗非鱼(Tilapia mossambica)饲料浸出液作为养殖有机污染物,分析比较4株酵母菌对其的降解率。结果显示,圆丘假丝酵母DY-11-1菌株对饲料浸出液的COD、总氮、总磷降解能力最高,9 d后分别由27.60 mg/mL、12.22 mg/mL和2.67 mg/mL下降到15.73 mg/mL、7.59 mg/mL和1.40 mg/mL,降解率分别为43.0%、37.8%和47.5%。表明DY11-1菌株对养殖有机污染物有较强的分解作用,在改善养殖水质方面具潜在的应用价值。Production of amylase,protease,lipase,and cellulose of four marine yeasts was compared using differential medium plate method,and the enzyme activities were also analyzed in this research.The results showed that Candida colliculosa DY11-1 could secrete amylase,protease and lipase,and their activities were 181.4,123.1 U/mL and 54.2 U/mL,respectively.Pichia sp DY11-6 and Candida parapsilosis DY07-1 could secrete amylase and protease.Their amylase activities were 108.1 and 96.5 U/mL,and their protease activities were 35.1 and 128.4 U/mL,respectively.Rhodotorula glutinis DY02-3 did not secrete the four extracellular enzymes.Feed extracts of Tilapia mossambica were used as aquaculture organic pollutants to compare the degradation ability of the four marine yeasts.The results showed that Candida colliculosa DY11-1 possessed the strongest degradation capacity.The concentration of COD,TN and TP in the tested water decreased from 27.60,12.22 and 2.67 mg/mL to 15.73,7.59 and 1.40 mg/mL after 9 days,respectively,with the degradation rates of 43.0%,37.8% and 47.5%.It indicated that DY-11 had potential application value in improving water quality of aquaculture.

关 键 词：海洋酵母菌 胞外酶 水产养殖有机污染物 净化 

分 类 号：Q17[生物学—水生生物学]