大豆下胚轴离体培养器官发生的研究  

STUDY ON ORGANOGENESIS OF SOYBEAN HYPOCOTYLS IN VITRO CULTURE

作　　者：胡卫静[1] 何丽君[1] 王明玖[2] 陈丽丽[2] 吕华龙[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019 [2]内蒙古农业大学生态环境学院,呼和浩特010018

出　　处：《内蒙古农业大学学报（自然科学版）》2013年第2期42-45,共4页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：内蒙古自然基金项目(2010MS0511);内蒙古应用技术研究与开发项目(20091402)

摘　　要：本试验选用内蒙古科尔沁草甸草原野生大豆(父本)与当地栽培大豆品种大白眉(母本)杂交后代的稳定株系9002的下胚轴作为供试外植体,探讨了大豆种子灭菌时间、激素类型等因素对大豆继代培养的影响。结果表明:①该大豆种子的最佳灭菌条件是:先70﹪乙醇杀菌1min,然后6﹪NaClO灭菌17min。②大豆诱导不定芽发育适宜培养基:MS+6-BA0.5 mg/L+KT1.0mg/L+IAA0.5 mg/L+蔗糖3﹪+琼脂0.8﹪。③适宜绿芽分化生根培养基:MS+NAA0.5 mg/L+蔗糖2﹪+琼脂0.8﹪为培养基。对大豆进行组织培养技术的成功有利于对加速良种繁育、优良种质资源保存、有益突变体筛选及大豆遗传操作等都具有重要意义。The test used in this assay Inner Mongolia Horqin meadow grassland wild soybean（male parent） with local the soybean of Da Baimei（female parent） stable line of hybrids.9002 of soybean seed sterilization time,hormone type and other factors affect soybean subculture.The results showed that： ① the soybean seed sterilization conditions： first with 70% ethanol sterilization 1min,and then the 6% Naclo sterilization 17min.② soybean induce adventitious buds culture conditions： MS ＋6-BA0.5 mg / L ＋ KT1.0mg / L ＋ IAA0.5 mg / L ＋ sucrose 3% ＋ agar 0.8%.③suitable for green bud differentiation rooting medium： MS ＋ NAA0.5 mg / L ＋ sucrose 2% ＋ agar 0.8%.For soybean on the success of the tissue culture technology help to accelerate breeding,excellent germplasm conservation,beneficial mutant screening and soybean genetic operation and so on all has the important meaning.

关 键 词：大豆 组织培养 器官发生 外植体 

分 类 号：S565.135[农业科学—作物学]