蛙血清去蛋白提取物对大鼠脑缺血再灌注损伤的保护作用  

The protective effects of deproteinized frog serum extract on cerebral ischemia-reperfusion injury of rats

作　　者：陈博[1] 

机构地区：[1]广东医学院东莞校区生理科学实验室,广东东莞523808

出　　处：《中国医药导报》2013年第16期19-21,24,共4页China Medical Herald

摘　　要：目的探讨蛙血清去蛋白提取物对大鼠脑缺血再灌注损伤的保护作用及其机制。方法将SD雄性大鼠72只,随机分为6组:假手术组、模型组、奥德金组(20 mg/kg)和蛙血清去蛋白提取物高(90 mg/kg)、中(30 mg/kg)、低(10 mg/kg)剂量组。采用线栓法栓塞大鼠大脑中动脉建立大鼠脑缺血再灌注损伤模型,从术前7 d开始,每天1次,造模当天于手术前1 h给药,再灌注后1 h给药1次。分别用化学比色法测定脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)含量;酶联免疫吸附法(ELISA)法测定肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)含量;原子吸收分光光度法测定Ca2+含量;干湿重法测定脑组织含水量。结果模型组大鼠脑组织SOD、GSH-Px含量明显降低,MDA、TNF-α、IL-1β、Ca2+含量和含水量明显升高(P<0.01);蛙血清去蛋白提取物能降低脑组织MDA、TNF-α、IL-1β、Ca2+含量和含水量,升高SOD、GSH-Px含量(P<0.05)。结论蛙血清去蛋白提取物防治脑缺血再灌注损伤的机制与提高抗氧化能力、抑制炎症起始因子、阻滞Ca2+超载有关。Objective To investigate the protective effect and mechanism of deproteinized frog serum extract on the rat model of cerebral ischemia-reperfusion injury. Methods 72 SD male rats into 6 groups were randomly divided into： sham operation group, model control group, Adegold group （20 mg/kg） and high dose group （90 mg/kg）, middle dose group （30 mg/kg）, low dose group （10 mg/kg） dose of deproteinized frog serum extract group. The model of cerebral is- chemia-reperfusion injury was established by inserting a nylon thread into the middle cerebral artery and then prac- tised administration, respectively once a day for seven days before the surgery, one hour before the surgery and one hour before reperfusion. Finally, the activity of brain tissue superoxide dismutase （SOD）, content of malondialdehyde （MDA） and glutathione peroxidase （GSH-Px） by means of chemical colorimetry were measured; the contents of tumor necrosis factor-α （TNF-α） and interleukin-lβ （IL-1β） of the brain were essayed by enzyme linked immunosorbent as- say （ELISA）; the content of Ca^2＋ by means of Atomic absorption spectrophotometry, the brain water content by means of wet and dry weight. Results The activity of SOD and GSH-Px of model control group rats decreased apparently, while the contents of MDA, TNF-α, IL-1β, Ca^2＋ and water in the brains increased remakably （P 〈 0.01）; deproteinized frog serum extract could significantly decrease the contents of MDA, TNF-α, IL-1β, Ca^2＋ and water in brain tissue, and re- markably increased the activity of SOD, GSH-Px （P 〈 0.05）. Conclusion The mechanism of deproteinized frog serum extract is by improve antioxidant capacity, inhibit inflammation initial factor and blockage Ca2＋ overload to protective of cerebral ischemia-reperfusion injury.

关 键 词：蛙血清去蛋白提取物 脑缺血再灌注 炎症因子 抗氧化 

分 类 号：R96[医药卫生—药理学]