γ-干扰素ELISA检测方法在广西牛结核病流行病学调查中的应用  

Epidemiological survey of bovine tuberculosis in Guangxi using interferon gamma ELISA detection

作　　者：徐贤坤[1] 熊毅[1] 韦达有 黄小武[3] 蓝纪 黄胜斌[1] 马琳[1] 刘棋[1] 

机构地区：[1]广西动物疫病预防控制中心,南宁530001 [2]桂林市动物疫病预防控制中心,广西桂林541001 [3]柳州市动物疫病预防控制中心,广西柳州545005 [4]南宁市动物疫病预防控制中心,南宁530001

出　　处：《南方农业学报》2013年第4期667-670,共4页Journal of Southern Agriculture

基　　金：广西科技攻关项目(桂科攻0719004-3F)

摘　　要：【目的】摸清广西水牛及奶牛结核病的流行情况，为制定净化广西牛结核病防制策略提供参考。【方法】2008～2011年采用皮内变态反应和γ-干扰素ELISA检测方法联合对广西奶牛及水牛开展结核病流行病学调查，共检疫5478头奶牛和1580头奶水牛。【结果】2008～2011年从广西南宁、柳州、北海、钦州、桂林等市累积检测出皮内变态反应阳性奶牛63头、水牛17头，阳性检出率分别为1.15％和1.08％，总体上广西奶牛及水牛结核病阳性率呈现逐年降低的趋势。通过采用自制水γ－干扰素EusA检测试剂盒和Bovigam牛结核分枝杆菌γ-干扰素检测试剂盒对牛结核病进行检测，发现γ-干扰素ELISA检测的牛结核病阳性率低于皮内变态反应，且准确度高。【结论】对牛群进行结核病检测时，先以变态反应检疫牛群，再结合γ-干扰素ELISA检测进行综合判断，更有利于牛结核病的检测与净化。[Objective]Epidemic survey was carried out to find out the prevalence of bovine tuberculosis in Guangxi. [Method]The tuberculin skin test （TST） and interferon gamma （IFN-g） ELISA assay were used to detect the bovine tuberculosis in Guangxi from 2008 to 2011. Totally 5478 cows and 1580 buffaloes had been quarantined for 4 years. [Result]During 2008-2011, 63 dairy cows （accounted for 1.15% of the total ） and 17 buffaloes （accounted for 1.08% of the total）were detected as positive in Nanning, Liuzhou, Beihai, Qinzhou and Guilin. Overall, the positive rate of tuberculosis in cow and buffalo decreased year by year. Through self-made box of interferon gamma assay and BovigamTM kit, it was found that the positive rate of bovine tuberculosis detected by interferon gamma assay which had high accuracy was lower than that by ]ST. [Conclusion]For better detection and purification of bovine tuberculosis, the herd should be quarantined by tuberculin skin test, and then tested by interferon gamma assay.

关 键 词：牛结核病 γ-干扰素ELISA 皮内变态反应 流行病学调查 阳性率 广西 

分 类 号：S858.23[农业科学—临床兽医学] S855.2[农业科学—兽医学]