^3H—3—叠氮基—N—正癸烷基水杨酰胺的合成  

Synthesis of ~3H-3-azidosalicyl-N-(n-decyl)amide

作　　者：吕斌[1] 武德柱[2] 陈世智[3] 徐建兴[1] 

机构地区：[1]中国科学院生物物理研究所,北京100101 [2]中国原子能科学研究院同位素研究所,北京102413 [3]中国医学科学院药物研究所,北京100050

出　　处：《原子能科学技术》2000年第2期132-135,共4页Atomic Energy Science and Technology

基　　金：国家自然科学基金 !(39570 1 93);国家科学技术部特别支持费资助项目

摘　　要：以 3 硝基水杨酸和正癸烷为原料 ,合成了研究线粒体呼吸链酶系中各泛醌结合蛋白的分子探针 ,即氚标记的 3 叠氮基 N 正癸烷基水杨酰胺 ,为进一步研究泛醌结合蛋白提供了有效工具。3 H 3 叠氮基 N 正癸烷基水杨酰胺的放射性比活度和放射化学纯度分别为 2 5 9TBq/mol和 98%A novel method for the synthesis of molecular probe of ubiquinone binding protein is described. With 3 nitrosalicylic acid and decylamine as initial compounds and under the existence of DCC, the 3 nitrosalicyl N (n decyl)amide is synthesized at room temperature. Then, 3 nitrosalicyl N (n decyl)amide is reduced by hydrogen with 5 % Pd/C as catalist to form 3 aminosalicyl N (n decyl)amide which is exchanged with tritium to be 3H 3 aminosalicyl N (n decyl)amide. At the temperature below 5 ℃, 3H 3 aminosalicyl N (n decyl)amide reacts with NaNO 2 and HCl, and the 3 diazosalicyl N (n decyl)amide is formed in an ice salt bath. As soon as the reaction is completed, NaN 3 is added to the mixture and stirred for 3 h at the temperature between 0～5 ℃ and in the dark, the molecular probe of studying ubiquinone binding protein, i. e., 3H 3 azidosalicyl N (n decyl)amide is produced.

关 键 词：合成 氚标记 3-叠氮基-N-正癸烷基水杨酰胺 

分 类 号：O628.11[理学—有机化学]