无角陶赛特羊和地方绵羊品种微卫星多态性分析及品种间杂种优势预测  

Analysis on Microsatellite Polymorphisms of Poll Dorset and Local Sheep and the Prediction of Their Crossbred Superiority

作　　者：罗惠娣[1] 毛杨毅[1] 张亚萍 刘平国 刘晓妮 周胜花[1] 郭慧慧[1] 赵鹏[1] 李俊[1] 张冠武[1] 

机构地区：[1]山西省农科院畜牧兽医研究所,太原030032 [2]山西省长治市畜牧局 [3]山西省兽药(饲料)监察所 [4]山西省生态畜牧产业管理站

出　　处：《中国草食动物科学》2013年第3期11-15,共5页China Herbivore Science

基　　金：山西省科技攻关基金(20120311024-1);(20090311033);(20080312002-5);山西省农业科学院育种基金(11yzgc019);国家绒毛用羊产业技术体系(CARS-40-14);山西省羊产业技术体系(2012-0803)

摘　　要：分析无角陶赛特羊和地方绵羊品种的遗传多样性及亲缘关系,以期为种质资源合理利用和保护提供理论依据。利用5个微卫星标记分析5个绵羊品种的遗传结构变异,根据等位基因组成及频率进行了群体遗传统计分析。结果表明:5个微卫星座位在5个绵羊品种中共检测到77.0个等位基因,各位点的等位基因数(Na)10.0～20.0个;5个绵羊品种平均多态信息含量(PIC)0.6152～0.737 3,平均基因杂合度(He)0.671 1～0.782 0。无角陶赛特羊与广灵大尾羊的标准遗传距离(DS)最大(0.6631),其余依次为晋中绵羊(0.6400)、乌珠穆沁羊(0.3712)、小尾寒羊(0.3352)。说明5个微卫星座位均为高度多态位点,可作为有效遗传标记用于绵羊品种的遗传多样性及品种间遗传关系分析;5个绵羊品种在5个微卫星位点遗传变异大、多态性丰富。据DS推测,无角陶赛特羊与广灵大尾羊杂交可望获得较大的杂种优势,与晋中绵羊的杂种优势次之,与乌珠穆沁羊的杂种优势较小,与小尾寒羊的杂种优势最小。In order to investigate the genetic diversity and kinship between Poll Dorset and local sheep breeds and provide a theory basis for proper germplasm resource protection and utilization, the population genetics was statistically analyzed basing on the allel composition and frequency of 5 microsatellite markers in 5 sheep breeds.The results showed that 77 alleles were detected totally in 5 microsatellite loci of the 5 breeds and each locus harbored 10-20 alleles; the average polymorphism information content （PIC） was 0.615 2-0.737 3, the average gene heterozygosity （He） was 0.671 1-0.782 0; the standard genetic distances （DS） of Poll Dorset with Guangling large-tailed sheep, Jinzhong sheep, Ujumqin sheep and Smalltailed Han sheep were 0.663 1,0.640 0,0.371 2 and 0.335 2 respectively. It indicated that all of the 5 microsatellite loci were highly polymorphic and could be used as effective genetic mark for analysis of genetic diversity and kinship of sheep breeds. In addition, according the DS, the hybrid superiority of Poll Dorset with local sheep breeds could be predicted.

关 键 词：无角陶赛特羊 地方绵羊品种 微卫星多态性 杂种优势 

分 类 号：S826.2[农业科学—畜牧学]