重组水蛭素与血栓前体关系的研究  

The study of the relationship of r-hirudin and prethrombus

作　　者：谭成[1] 万卫星[1] 张荣军[1] 张莲芬[1] 韩玉珉[2] 金坚[1] 

机构地区：[1]江苏省原子医学研究所核医学国家重点实验室,无锡214063 [2]中国科学院生物物理研究所

出　　处：《核技术》2000年第5期343-346,共4页Nuclear Techniques

基　　金：卫生部科研基金!96-1-344

摘　　要：氯胺－Ｔ法碘标水蛭素，采用固相饱和法、固相竞争法分析水蛭素与凝血酶－纤维蛋白原复合物的亲和力及定量结合关系，并分析水蛭素－凝血酶－纤维蛋白原复合物形成的时间反应动力学。结果显示，水蛭素可特异地与凝血酶－纤维蛋白原复合物形成三元复合物，它们的分子配比为１４∶１４∶１（水蛭素：凝血酶：纤维蛋白原）。生理Ｃａ^（２＋）浓度是它们的最佳结合环境，水蛭素与凝血酶－纤维蛋白原复合物结合的亲和常数Ｋ_ａ为９．７２ ×１０~６Ｌ／ｍｏｌ；其结合反应可在３０－４５ｍｉｎ达到平衡，提示水蛭素有作为血栓显像配基的可能性。^(125)I-rhirudin was prepared by chloramine-T method. The affinity of rhirudin with thrombin-fibrin complex was analyzed with solid phase saturation method and competitive method. The kinetics of the rhirudin-thromb in-fibrin complex formation was studied too. Results indicated that rhirudin was able to bind with thrombin-fibrin complex as a tenary complex, but not directly with fibrinogen. The molecular binding ratio was 14:14:1 for rhirudin:thrombin:fibrinogen. The affinity constant of rhirudin with thrombin-fibrin complex was 9.72×10~6L/mol, and the physiological Ca^(2+) concentration was their optimal binding environment. The binding reaction of rhirudin with thrombin-fibrin complex could reach the equilibrium in 30 to 45 minutes. All these results indicate that rhirudin is hopefully a new ligand for thrombotic image.

关 键 词：水蛭素 凝血酶 碘标记物 血栓病 放射诊断 

分 类 号：R817.43[医药卫生—影像医学与核医学]