^12C^6＋重离子辐射下彩色马蹄莲生理生化和抗病性的变化及ISSR多态性分析  

Physiological and Biochemical and Resistance Changes and Issr Polymorphic Analysis Exposed to ^(12)C^(6+) Heavy Ion Radiation on Calla Lily

作　　者：陈臻[1,2] 徐秉良[1] 蒲崇建 徐琼[3] 田谷[1] 

机构地区：[1]时肃农业大学草业学院植物病理系/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]甘肃省植保植检站,甘肃兰州730020 [3]甘肃省兰州市农业科学研究所,甘肃兰州730000

出　　处：《核农学报》2013年第5期552-556,共5页Journal of Nuclear Agricultural Sciences

基　　金：兰州市科技局高新技术产业化项目(2008-1-171)

摘　　要：采用12C6+重离子辐射彩色马蹄莲,研究12C6+重离子辐射下彩色马蹄莲发生变异的生理生化机制。结果表明:辐射剂量与球茎萌发率、株高之间均呈极显著负相关,与丙二醛(MDA)含量呈极显著正相关,叶片组织内过氧化氢酶(CAT)、过氧化物酶(POD)活性和植株抗病性随着辐射剂量的增加均呈现先升高后降低的趋势,辐射的适宜剂量为10～20Gy。同时应用ISSR技术对彩色马蹄莲进行检测分析,发现22条ISSR引物能扩增出121条谱带,其中55条为多态性谱带,多态性比率为45%,辐射后球茎与对照差异表现为增加或减少了扩增片段。分析表明:12C6+重离子辐射彩色马蹄莲表现的影响主要为损伤和抑制效应,但适宜剂量范围内12C6+重离子辐射可作为彩色马蹄莲育种的手段之一。Physiological and biochemical changes and ISSR Polymorphic of calla lily caused ion radiation were studied. The results showed that bulb germination rate and plant hei exposure to ^12C^6＋ heavy- had significant negative correlation with radiation dose, while MDA content had high significant positive correlation with radiation dose. With increasing radiation dose, the activities of CAT, POD and resistance showed a trend of decrease after an initial increasing. Optimum doses of irradiation were 10 - 20Gy. ISSR molecular marker of the control and variant plants induced by the ^12C^6＋ heavy-ion radiation suggested that 121 bands were amplified with 22 ISSR primers among two calla lily varieties, 55 bands were polymorphic and the polymorphism rate reached to 45% , the ^12C^6＋ heavy-ion radiation could cause mutation of genome DNA in calla lily. It is suggested that effect of irradiation on calla lily plant was damage and suppression. Optimum doses of irradiation of ^12C^6＋ Heavy ion might be applied for breeding method on Calla lily.

关 键 词：彩色马蹄莲 12C6+重离子辐射 生理生化特性 抗病性 ISSR 

分 类 号：S682.264[农业科学—观赏园艺]