甘薯种质资源的SRAP鉴定及遗传多样性分析  

Genetic Diversity among Main Sweetpotato Germplasm Resources using SRAP Markers

作　　者：张凯[1,2] 罗小敏 蒋玉春[1,2] 吴正丹[1,2] 王季春[1,2] 唐道彬[1,2] 

机构地区：[1]西南大学农学与生物科技学院,重庆400716 [2]重庆市甘薯工程技术研究中心,重庆400716 [3]重庆市农业技术推广总站,重庆401121

出　　处：《核农学报》2013年第5期568-575,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31101192);中央高校基本科研业务费专项(XDJK2011C004);重庆市科技攻关项目(cstc2010AB1053);西南大学博士基金(SWU110031)

摘　　要：为了拓宽甘薯育成品种的遗传背景,筛选优良亲本,提高育种效率,本研究利用SRAP标记对48份主要甘薯种质资源进行了遗传多样性分析。结果表明,随机选用的37对SRAP引物中29对引物具有多态性,引物多态性比率为78.4%,共获得126条多态性谱带,平均每对引物产生4.3条多态性谱带,表现出较高的多态性。48份种质材料的SRAP遗传距离为0.037～0.601,当遗传距离L1=0.46时,48份材料被聚为6个类群,包括1个复合大类群和5个独立类群,其中第Ⅰ复合大类群又包括7个亚类群,聚类结果与系谱吻合性较好。采用5个重要农艺性状对供试材料进行了聚类分析,L1=3.20时,48份材料也可被聚为6个类群,聚类结果与依据SRAP标记聚类分析的结果差异较大。甘薯种质资源间的遗传差异与地理来源无必然联系。To broaden the genetic background of sweetpotato cultivars, screen the excellent parents for breeding, and improve breeding efficiency, the genetic diversity of 48 main sweetpotato germplasm resources were assessed using SRAP markers in this study. The results indicated that 29 of the 37 randomly selected primer pairs amplified polymorphic bands among the 48 sweetpotato germplasm with the polymorphism ratio of 78.4%. A total of 126 polymorphic bands were generated, and of these, 4.3 polymorphic bands per primer pairs in average could be detected. The genetic distance based on SRAP markers ranged from 0. 037 to 0. 601 among the different germplasm. At the genetic distance of 0. 46, 48 germplasm were classified into six main groups, which included one composite group and five independent ones, and the composite group could be further divided into seven subgroups. The cluster result was consistent with the known pedigree of these varieties. Based on the cluster analysis of 5 important agronomics traits, 48 germplasm were classified into six main groups when the L1 was set to 3.20. The phenotypic correlation calculated based on the agronomic characteristics showed difference from the cluster result assessed by SRAP markers. The positive relationship between the genetic differences and the geographic origin were not observed.

关 键 词：甘薯 遗传多样性 聚类分析 SRAP 农艺性状 

分 类 号：S531[农业科学—作物学]