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机构地区:[1]东北农业大学生命科学学院,黑龙江哈尔滨150030 [2]桂林理工大学化学与生物工程学院,广西桂林541004
出 处:《核农学报》2013年第5期687-693,共7页Journal of Nuclear Agricultural Sciences
基 金:东北农业大学科学研究基金(2010RCB56);广西自然科学基金(桂科回0731021)
摘 要:以水稻无侧根突变体RM109为材料,对其无侧根特性的其它伴随性状进行了研究,旨在揭示遗传突变引起的生理生化变化及其生物学特性。结果表明:突变体的子粒宽、厚、百粒重、百粒谷物量均小于野生型,子粒和发芽第5天的百粒淀粉含量分别是野生型的135%和157%,萌发第4天的幼苗根系琥珀酸脱氢酶(SDH)比活性是野生型的8%;突变体经1μmol·L-1的AgNO3处理后对ACC、H2O2、2,4-D及TIBA都表现出耐性,但对照组突变体(无Ag+处理)对1-氨基环丙烷-1-羧酸(ACC)和H2O2的耐性低于野生型、对2,4-二氯苯氧乙酸(2,4-D)及三碘苯甲酸(TIBA)的耐性高于野生型。In this paper, the lateral rootless mutant (RM109) was applied to study others accompany-linked traits except the rootless character in order to reveal variation of the physiological and biochemical aspects induced by genetic mutation. Compared to the wild type, some physiological indexes of RM109 were decreased, including grain width, grain thickness, hundred-grain weight, and the amount of hundred-grain. In RM109, Starch content of one hundred grains was 135% of wild type at the seed period and 157% of wild type after germinated for 5 days, while SDH specific activity of root was 8% of wild type after germinated for 4 days. After treated with 1 μmol. L-1 AgNO3. RM109 can be tolerant to ACC, H2O2 , 2, 4 - D, and TIBA, but the tolerance to ACC and H202 with non-Ag+ was lower than that of wild type, and the tolerance to 2, 4 - D and TIBA with non-Ag+ was higher than that of wild type.
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