枯草芽孢杆菌B26-10抑菌物质的稳定性与分离  

Purification of Antifungal Metabolites of Bacillus subtilis B26-10 against Sapstain Fungi

作　　者：孙薇[1] 常建民[1] 张柏林[1] 韩丽[1] 王雨[1] 

机构地区：[1]北京林业大学,北京100083

出　　处：《东北林业大学学报》2013年第6期72-75,共4页Journal of Northeast Forestry University

基　　金：高等学校博士学科点专项科研基金(20110014110001)

摘　　要：分析了生防菌株枯草芽孢杆菌B26-10抑菌物质部分理化性质,在此基础上对抑菌物质进行分离。主要结论如下:枯草芽孢杆菌B26-10抑菌物质经过调节pH值去除杂蛋白、(NH4)2SO4沉淀、Sephadex G-75层析提取后,SDS-PAGE检测为单一条带,相对分子质量约为63 500。高温、强酸、强碱会使抑菌物质失活,抑菌物质对常规有机溶剂具有良好的稳定性。Bacillus subtilis B26-10 exhibits antifungal activity against sapstain fungi. The antifungal metabolites were purified from its crude extracts through adjustment of pH, precipitation with （NH4 ）2SO4 and gel filtration chromatography. The SDS-PAGE inspection shows Bacillus subtilis B26-10 is one band with the molecular mass of 63 500. Antifungal metabolites will lost antifungal activity with high temperature, strong acid and strong alkali, while keep stable with organic solvent.

关 键 词：木材蓝变 枯草芽孢杆菌 抑菌物质 分离 

分 类 号：S781.52[农业科学—木材科学与技术]