尖孢镰孢菌番茄专化型的鉴定研究  

Study on Identification of Tomato Biotyoe Fusarium oxysporum f.sp.lycopersici

作　　者：王小皙[1] 高增贵[1] 张硕[1] 周艳波[1] 王敏[1] 孔婷婷[1] 

机构地区：[1]沈阳农业大学植物免疫研究所,辽宁沈阳110866

出　　处：《北方园艺》2013年第11期100-103,共4页Northern Horticulture

基　　金：辽宁省科技攻关资助项目(2011214002);沈阳市科技攻关专项资助项目(F12-119-3-00)

摘　　要：以27株镰孢菌菌株为试材,采用菌株形态学观察、rDNA-ITS序列分析以及寄主专化型鉴定相结合的方法,鉴定了尖孢镰孢菌番茄专化型。结果表明:经形态学和rDNA-ITS测序筛选,最后筛选出10株尖孢镰孢菌。将尖孢镰孢菌交叉接种到不同寄主植物,确定菌株编号YH070001和DL070009的镰孢菌为尖孢镰孢菌番茄专化型。与单一的传统方法相比,ITS rDNA方法有着较高的灵敏度,所以采取形态学、现代分子生物学以及传统交叉接种相结合的鉴定方式,能够使菌株专化型的鉴定更加准确、完善。Taking 27 Fusarium strains as materials,F.oxysporum f.sp.lycopersici was identified using morphology observation and rDNA-ITS sequencing.The results showed that after selected by morphology and rDNA-ITS sequencing,10 F.oxysporum strains were identified by traditional method of specialized form as cross inoculation.Thus N0.YH070001 and DL070009 were confirmed F.oxysporum f.sp.lycopersici.Compared with single traditional method,rDNA-ITS sequencing has higher sensitivity.To test the accuracy and feasibility of this identification method,morphology observation,rDNA-ITS sequencing and traditional method of specialized form identification were used in this study.

关 键 词：尖孢镰孢菌 番茄枯萎病 RDNA-ITS 交叉接种 

分 类 号：S436.412.19[农业科学—农业昆虫与害虫防治]