吸入糖皮质激素对恐惧应激哮喘大鼠的影响  

Effects of inhaled glucocorticoid on fear stress-induced rat models with asthma

作　　者：陈强[1] 代佳佳[1] 杨文萍[2] 柯江维[3] 刘志强[3] 周红平[3] 刘建梅[1] 肖鲁闽[4] 

机构地区：[1]江西省儿童医院呼吸科,南昌330006 [2]江西省儿童医院病理科,南昌330006 [3]江西省儿童医院检验科,南昌330006 [4]南昌大学第二附属医院动物实验室

出　　处：《中华实用儿科临床杂志》2013年第9期662-665,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：江西省科技支撑计划项目（20111BBG70020-3）

摘　　要：目的探讨负性情绪对卵清蛋白（OVA）致敏诱发哮喘大鼠细胞因子、NK细胞、神经激肽酶A受体（NK2-R）的影响及吸入糖皮质激素的干预效果。方法雄性sD大鼠32只分为4组，对照组（A组）、致敏组（B组）、恐惧应激致敏组（C组）、恐惧应激致敏＋吸入糖皮质激素干预组（D组），给予OVA建立致敏大鼠模型；强迫浸水模拟恐惧应激环境干预；面罩雾化吸入糖皮质激素进行干预。采用四导生理记录仪测定各组大鼠呼吸道峰压值，HE染色观察肺组织呼吸道炎症，流式细胞仪测定外周血NK细胞数量，ELISA法测定血清IL-4、IFN-γ的水平，荧光定量PCR（RT-PCR）法测定大鼠肺组织中NK2-RmRNA水平。结果C组血清IL4水平及呼吸道峰压值高于B组及A组（P均〈0．05），而血清IFN-γ水平及外周血NK细胞数量较B组及A组降低（P均〈0．05）；肺组织NK2-RmRNA水平c组较B组高（P〈0．05）；D组呼吸道峰压值、血清IL-4水平及肺组织NK2一RmRNA水平均较C组降低（P均〈0．05），血清IFN-γ水平及外周血NK细胞数量D组较C组增高（P均〈0．05）。结论负性情绪能加重哮喘大鼠的呼吸道炎症，不仅影响Th1／Th2型细胞因子平衡，还能在减少外周血中NK细胞，增加肺组织NK2-RmRNA的表达等方面影响哮喘大鼠的呼吸道炎症；吸入糖皮质激素不仅能纠正Th1／Th2细胞因子失衡，降低肺组织NK2一RmRNA的表达，还能增加外周血NK细胞数量。Objective To determine the effect of negative moods on ovalbumin（OVA） in allergic rat models by examining cytokines and natural killer（NK） cells in peripheral blood, neurokinin A receptor（NK2-R） in the lung and observe the differentials during inhaled glucocorticoid inhalation. Methods Thirty-two healthy male SD rats were divided into 4 groups, group A as the control group, group B as the allergic group ,group C as the fear-stressed-allergic group, group D as the glucocorticoid inhalation treatment and fear-stress-allergic group. The allergic rat models were es- tablished by OVA. Forced swimming test was performed at the same time in group C and group D, and glucocorticoid in- halation was given at the same time as OVA challenge in group D. Airway peak pressure was measured with four-lead graph system, HE staining was used to observe the inflammation of the lung , the level of NK cells in peripheral blood was detected by flow cytometry, the cytokines IL-4, IFN-γ levels in the serum were determined by enzyme-linked immuno sorbent assay,NK2-R mRNA level was evaluated by transcription-quantitative. Results The levels of IL-4 and airway peak pressure in group C were higher than those in group B and group A （ all P 〈 0.05 ） ; the level of IFN-γ in the serum and the number of NK cells in peripheral blood in group C diminished compared with group B and group A （ P 〈 0.05） ; the expression of NK2-R mRNA in the lungs in group C was higher than that in group B（P 〈0.05） ; while in group D airway peak pressure, IL-4 level in the serum and the expression of NK2-R mRNA in the lungs were lower than those in group C（ all P 〈0.05 ） ; the levels of IFN-γ in the serum and the number of NK cells in peripheral blood in group D were higher than those in group C （ all P 〈 0.05 ）. Conclusions Negative moods may aggravate the inflammation reactions in the respiratory tract of the asthmatic rats, which may affect the balance between Th1/Th2 cytokines, reduce the number of NK cells in the periph

关 键 词：支气管哮喘 负性情绪 糖皮质激素 大鼠 

分 类 号：R5[医药卫生—内科学]