不同淋巴细胞激活物对支气管哮喘患儿Foxp3表达的影响  

Influence of different T cell activators on the expression of Foxp3 in bronchial asthma

作　　者：王炜[1] 李芳[1] 段国威[1] 吴晓玲 宋青 

机构地区：[1]河北医科大学第二医院小儿内科,石家庄050000 [2]石家庄市井陉县医院儿科 [3]石家庄市深泽县医院儿科

出　　处：《中华实用儿科临床杂志》2013年第9期666-668,共3页Chinese Journal of Applied Clinical Pediatrics

基　　金：2011年河北省科技支撑计划项目（10276151）

摘　　要：目的探讨不同淋巴细胞激活物对支气管哮喘患儿T淋巴细胞Foxp3表达的影响。方法选择对户尘螨过敏的轻度持续支气管哮喘患儿30例，分离所有入选患儿的外周血淋巴细胞，将每例患儿的淋巴细胞分为2组，一组予户尘螨浸出液刺激（户尘螨组），另一组予佛波酯（PMA）刺激（PMA组），并将2组淋巴细胞培养48h后，应用流式细胞仪检测CD4＋CD25＋T淋巴细胞、CD4＋CD25＋Foxp3＋T淋巴细胞和CD4＋CD25＋Voxp3＋IL-10＋T淋巴细胞水平的变化。结果户尘螨组CD4＋CD：5＋T淋巴细胞水平[（4．59±1．10）％]显著低于PMA组[（41．24±7．95）％]（P〈0．0001）；户尘螨组CD4CD25＋Foxp3＋T淋巴细胞水平[（18．08±1．82）％]显著低于PMA组[（90．26±3．63）％]（P〈0．0001）；户尘螨组CD4＋CD2s＋Foxp3＋IL-10＋T淋巴细胞水平[（37．62±3．23）％]与PMA组[（38．33±3．95）％]比较差异无统计学意义（P〉0．05）。结论非特异性淋巴细胞激活物可使支气管哮喘患儿T淋巴细胞Foxp3过高表达。Objective To explore the influence of different T cell activators on the expression of Foxp3 in bronchial asthma. Met-hods Peripheral blood monouclear（PBMCs） were isolated from the 30 children suffering from mild persistent asthma allergic to house dust mite （HDM）. PBMCs of those children were divided into 2 groups. One group was stimulated with HDM extracts （ HDM group） and the other was stimulated with phorbol-12-myristate-13-acetate（PMA） extracts （ PMA group）. After 48 hours of in vitro stimulation with HDM extracts or PMA extracts, the levels of CD4^＋ CD25^ ＋ T cells, CD4^＋ CD25^ ＋ Foxp3^＋ T cells and CD4^＋ CD25^ ＋ Foxp3 ^＋ IL-10 ^＋ T cells were measured by flow cytometry. Results The levels of CD4^＋ CD25^ ＋ T cells significantly decreased in the HDM group[ （4.59 ±1.10） % ] compared with those in the PMA group [ （41.24 ±7.95 ） % ] （ P 〈 0.0001 ）. The levels of CD4^＋ CD25^ ＋ Foxp3 ^＋ T cells significantly decreased in the HDM group [ （ 18.08 ±1.82） % ] compared with those in the PMA group [ （90.26 ±3. 63） % ] （P 〈 0. 0001 ）. The levels of CD4^＋ CD25^ ＋Foxp3^ ＋ IL-10 ^＋ T cells had no statistical significance in the HDM group [ （37.62 ± 3.23 ） % ] compared with those in the PMA group [ （38.33 ±3.95 ） % ] （ P 〉 0.05 ）. Conclusion Nonspecific T cell activators earl increase the expression of Foxp3 in bronchial asthma.

关 键 词：佛波酯 户尘螨 支气管哮喘 Foxp3 调节性T淋巴细胞 

分 类 号：R72[医药卫生—儿科]