穿心莲内酯磺化物及布地奈德对支气管哮喘小鼠白细胞介素-17、白细胞介素-23表达的影响  

Effects of andrographolide and budesonide on the expressions of interleukin-17 and interleukin-23 in asthmatic mice model

作　　者：蒋菲[1] 李艳华[1] 王美怡[1] 张建华[1] 

机构地区：[1]上海交通大学附属第六人民医院儿科,200233

出　　处：《中华实用儿科临床杂志》2013年第9期669-672,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：基金项目：上海交通大学附属第六人民医院院内科研基金（院内-1142）

摘　　要：目的观察穿心莲内酯磺化物及布地奈德（BUD）对支气管哮喘小鼠嗜酸性粒细胞（EOS）、中性粒细胞、IL-17、IL-23表达的影响。方法BALB／c小鼠32只，随机分为哮喘组、穿心莲内酯磺化物组、BUD组、对照组，每组8只。注射和雾化吸入卵清蛋白（OVA）复制哮喘动物模型，以穿心莲内酯磺化物、BUD进行干预，计数支气管肺泡灌洗液（BALF）中白细胞总数、中性粒细胞和EOS数，ELISA检测BALF及外周血中IL-17、IL-23的表达，实时荧光定量PCR法检测肺组织中IL-17mRNA和IL-23mRNA的表达。结果穿心莲内酯磺化物组、BUD组的BALF中白细胞总数分别为（111．98±3．28）×10^7／L和（64．16±36．75）×10^7／L，EOS计数分别为（131．29±4．89）×10^7／L及（82．40±11．48）×10^7／L，均较哮喘组[白细胞总数（208．78±18．52）×10^7／L及EOS计数（106．80±13．90）×10^7／L]明显降低（P均〈0．05）。穿心莲内酯磺化物组与BUD组比较，BALF及外周血的IL-17蛋白水平差异无统计学意义（P〉0．05）。BUD组BALF中IL一23蛋白[89．92（113．87-67．79）ng／L]水平较哮喘组[93．30（173．39-42．95）ng／L]显著下降（P〈0．05）。穿心莲内酯磺化物组、BUD组IL-23mRNA表达分别较哮喘组明显受抑制（P〈0．05），但IL-17mRNA表达在干预组和哮喘组间差异无统计学意义（P〉0．05）。结论穿心莲内酯磺化物及BUD均能抑制哮喘小鼠BALF中自细胞总数及EOS计数和肺组织IL-23mRNA表达，但对中性粒细胞数和IL-17基因表达的抑制作用不明显。Objective To evaluate the effects of andrographolide and budesonide （BUD） on eosinophil （EOS） counts and neutrophilic and the expressions of IL-17,IL-23 in asthmatic mice. Methods BALB/c mice were randomly divided into asthmatic group,andrographolide group,BUD group and control group,8 mice in each group. The mice were sensitized and challenged by ovalbumin（OVA） to establish asthmatic mice model. Two groups of mice were treated with andrographolide and BUD, respectively. Twenty-four hours after the last administration, inflammatory cell count,cell classification in bronchoalveolar lavage fluid （BALF） and lung histological examination were carried out. Wright-Giemsa was used to count total,EOS,neutrophilie cells of BALF. Meanwhile,ELISA was used to detect the con- centrations of IL-17 and IL-23 of BALF and peripheral blood. The expressions of IL-17 mRNA and IL-23 mRNA of the lung tissue were detected by quantitive Real-time PCR. Results Total cell and EOS counts in BALF of 2 treatment groups[ （111.98±3.28）×10^7/L and （64.16 ±36.75） ×10^7/L, （131.29±4.89） ×10^7/L and （82.40± 11.48） ×10^7/L] decreased significantly compared with asthma group [ （208.78±18.52） ×10^7/L and （ 106.80 ± 13.90） ×10^7/L] （all P 〈 0.05 ）. IL-17 protein levels in BALF and peripheral blood were not significantly different between ad-ministration groups（P 〉 0.05）. IL-23 protein levels in BALF of BUD treated group[ 89.92 （ 113.87 - 67.79） ng/L] were significantly lower than asthma group[ 93.30 （ 173.39 -42.95） ng/L] （P 〈 0.05）. IL-23 mRNA expressions in treatment groups[2.14 （8.54 -0.22） and 1.18 （4.58 -0.25） ] were significantly suppressed when compared with asthmatic group[5.08 （10.14 -1.42 ） ] （P 〈 0.05 ） ,while 1L-17 mRNA expressions had no significant difference be- tween them （ P 〉 0.05 ）. Condnsions Andrographolide and BUD can suppress the infiltration of total inflammatory cells and EOS expression IL-23 mRNA in BALF. They had no signific

关 键 词：哮喘小鼠模型 穿心莲内酯磺化物 布地奈德 白细胞介素-17 白细胞介素-23 

分 类 号：R28[医药卫生—中药学]