原生质体紫外诱变高通量选育盐霉素高产菌  

High-throughput Screening of High Salinomycin-producing Strains with UV Treatment on Protoplasts

作　　者：贾永峰[1] 梁剑光[1,2,3] 王永红[1] 郝玉有[1] 储炬[1] 

机构地区：[1]华东理工大学生物反应器国家重点实验室,国家生化工程技术研究中心,上海200237 [2]常熟理工学院生物与食品工程学院,江苏常熟215500 [3]浙江升华拜克生物股份有限公司,浙江湖州313220

出　　处：《中国医药工业杂志》2013年第6期560-564,共5页Chinese Journal of Pharmaceuticals

基　　金：国家重大科学仪器设备开发专项(2012YQ150087);江苏省高校自然科学基金项目(11KJB180001)

摘　　要：以白色链霉菌(Streptomyces albus)S-610为出发菌株,利用溶菌酶水解菌丝体细胞壁获得原生质体,然后进行紫外诱变,并通过高通量筛选再生突变株。结果显示,当UV照射原生质体20 s时,细胞正突变率最大为24.5%,此时致死率为86.4%。经高通量筛选和摇瓶验证,最终筛得3株遗传稳定的高产菌S-612、S-620和S-622,产盐霉素能力较出发菌株S-610分别提高了57.6%、45.5%和69.7%。The protoplasts of Streptomyces albus S-610 were prepared by losozyme hydrolyzation and then were treated by UV. Regenerative mutants were screened by high-throughput screening procedure. Positive mutation rate of cells reached 24.5% of maximum value and lethality rate was 86.4% when the protoplasts were treated by UV for 20 s. Finally, 3 high producers with genetic stability, designated S-612, S-620 and S-22, respectively, were successfully obtained after high-throughput screening and validation in shake flasks. Compared with the original strain S-610, their salinomycin productions were increased by 57.6 %, 45.5 % and 69.7 %, respectively.

关 键 词：盐霉素 白色链霉菌 原生质体技术 高通量筛选 

分 类 号：TQ969[化学工程]