六味地黄丸影响2型糖尿病大鼠肝脏SREBP-1和ACC表达的实验研究  

The effect of Liuwei Dihuang pills on the expression of SREBP and ACC genes in the liver of type 2 diabetic rats

作　　者：杜华[1] 薛耀明[1] 朱波[1] 钱毅[1] 

机构地区：[1]南方医科大学南方医院内分泌代谢科,广州市510515

出　　处：《实用医学杂志》2013年第11期1741-1743,共3页The Journal of Practical Medicine

基　　金：广东省中医药局科研课题(编号:2009454)

摘　　要：目的:观察六味地黄丸对2型糖尿病大鼠肝脏脂代谢调控相关基因固醇调节元件结合蛋白(SREBP)和乙酰辅酶A羧化酶(ACC)表达的影响。方法 :以LETO鼠为正常组,糖尿病大鼠模型OLETF鼠分为不加药的对照组以及加药的干预组,制作病理切片观察各组肝脏组织形态学改变,RT-PCR检测SREBP-1、ACC在各组大鼠肝脏组织中的表达情况。结果:与对照组相比,干预组大鼠肝脏细胞索结构完整,仅见轻度肝细胞脂肪变性。RT-PCR检测显示SREBP-1在正常组表达稳定,而在对照组和干预组的表达均随周龄增加而增加(P<0.01),但干预组SREBP-1的表达显著低于对照组(P<0.01)。ACC在对照组中的表达亦随周龄增加而明显增加,但其在正常组及干预组中的表达要显著低于对照组(P<0.01)。结论:六味地黄丸能降低糖尿病大鼠模型肝脏中SREBP mRNA和ACC mRNA的表达,可能通过减轻脂肪变性,改善糖尿病肝脏的脂代谢紊乱。Objective To study the effect of Liuwei Dihuang pills on the expression of SREBP and ACC in the liver of type 2 diabetic rats. Method Long Evans Tokushima Otsuka （LETO） rats served as an animal model of normal rat, and Otsuka Long-Evans Tokushima Fatty （OLETF） rats were established as an animal model of type II diabetic rats. OLETF rats were divided into two subgroups： an intervention group taking Liuwei Dihuang pills and a control group which were not to receive any medication. Each group was assessed the morphological changes and detected SREBP- 1 and ACC expressions in the liver tissue by RT-PCR. Results For the structural integrity of the liver cell, mild steatosis was found in the intervention group. RT-PCR analysis indicated that the expression of SREBP in the normal group was stable, whereas increased with weeks of age in the control group and intervention group （P 〈 0.01 ）. Moreover, the expression of SREBP in the intervention group was significantly lower than that in the control group （P 〈 0.01 ） The expression of ACC in the control group was also clearly increased with the weeks of age and significantly greater than those in the normal group and intervention group （P 〈 0.01 ）. Conclusion Liuwei Dihuang pills could reduce the expression of SREBP mRNA and ACC mRNA in the liver of OLETF rats. These findings indicated that the effect of Liuwei Dihuang pills could reduce lipid synthesis and improve the lipid metabolism in diabetic liver.

关 键 词：糖尿病 2型 六味地黄丸 固醇调节元件结合蛋白 乙酰辅酶A羧化酶 

分 类 号：R285.5[医药卫生—中药学]